FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715146
Sequences flagged as poor quality0
Sequence length75
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCT280883.927589611072425No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTG102111.4278203331907051No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCG94831.3260229379735047No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGT87351.221428911019568No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG86051.2032508047307822No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA66970.9364521370461416No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC62680.8764643862931486No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT49450.691467196908044RNA PCR Primer, Index 41 (95% over 22bp)
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTG47840.6689543114273169No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGG44630.6240683720526997No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTT39560.5531737575264352No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG32270.451236530722398No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT27960.3909691167957312No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG25650.35866802023642724No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCG25340.354333241044486No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTT22010.307769322627827No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT21440.29979892217812865No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTG20650.2887522268180204No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA20430.2856759319076105No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG19260.26931563624770327No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTC19050.2663791729241302No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT18770.2624638884926994No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTA18310.25603163549820596No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTT17630.24652308759330263No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC16680.23323908684380534No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTG15430.2157601384892036No Hit
CGTCAGATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGA15310.21408215944716183No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC14610.2042939483685849No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA13860.19380657935582385No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCC13760.1924082634874557No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGC13160.18401836827724688No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTA12210.1707343675277496No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGA11610.16234447231754076No Hit
GCAACTCATCCTTTGCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT11470.16038683010182536No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCC10860.15185710330477972No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGGCG10700.1496197979153907No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTT10070.14081040794467145No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGGCGTG9820.13731461827375108No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG9610.13437815495017802No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTC9330.13046287051874722No Hit
GGTTTGAGGCCTGTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATG9150.12794590195568456No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTTTGCCGTG8940.12500943863211147No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG8840.12361112276374335No Hit
GGTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAG8500.11885684881129167No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAA8260.11550089072720816No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGT8190.11452206961935045No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTGTT8160.11410257485884003No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC8050.11256442740363505No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGGCGTG7950.11116611153526691No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTG7870.1100474588405724No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA7820.10934830090638835No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTTTGCCGTG7760.10850931138536746No Hit
GTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGA7760.10850931138536746No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC7740.10822964821169384No Hit
GTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA7650.10697116393016252No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG7430.10389486901975262No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGGCTT7420.10375503743291578No Hit
CGTCAGATGTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCC7300.10207705839087403No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGT7270.10165756363036359No Hit
GTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC7260.10151773204352679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)