FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592751
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCT310335.235419256989866No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC169522.8598855168527764No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA110531.864695293639319No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT91351.5411192895499122TruSeq Adapter, Index 12 (95% over 22bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG89661.5126081609309812No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG61691.0407405470425186No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTG53750.9067888540044639No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGT51190.8636003988183909No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC46520.7848152090844216No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC46190.7792479472830919No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG39830.6719516289301916No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT36340.613073617758553No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTT33410.5636430811588677TruSeq Adapter, Index 12 (96% over 28bp)
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG33370.5629682615465853No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTC26050.43947627249890764No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAAT25310.4269921096716834No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAA24510.4134957174260355TruSeq Adapter, Index 12 (96% over 29bp)
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT23960.40421694775715267No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC18640.31446593932359457TruSeq Adapter, Index 12 (95% over 23bp)
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCTT18470.3115979559713944No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT17820.30063213727180554TruSeq Adapter, Index 12 (95% over 22bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG17500.2952335803735464No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGT17260.29118466269985205No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCT16610.28021884400026315No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTA16420.2770134508419218No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTA15450.2606490752440738No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTG15210.25660015757037946No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTG14870.2508641908659791No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG14310.24141671629402564No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTG13490.22758291424223662No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTT13170.2221843573439775No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCG11620.19603509736803482No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT11600.19569768756189362TruSeq Adapter, Index 12 (95% over 24bp)
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATAT11010.18574409828072833No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC10530.17764626293333963TruSeq Adapter, Index 12 (95% over 23bp)
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG10230.1725851158412217No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA9080.15318405198810292No Hit
GCAACTCATCCTTTGCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAAT9070.15301534708503234No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG8580.14474880683457303No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT8580.14474880683457303No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCTTCTGCTT8500.14339916761000826Illumina PCR Primer Index 12 (96% over 26bp)
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATG8150.13749449600253733No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATATCGTATGCCGTCTT7780.1312524145889252No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG7700.12990277536436043No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC7640.12889054594593682No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCG7610.12838443123672502No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCG7560.12754090672137203No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTC7460.12585385769066607No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTG7040.11876825176170096TruSeq Adapter, Index 12 (95% over 22bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCG6760.11404451447572421No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC6640.11202005563887704No Hit
CCAGCTCCTCACCAACACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA6440.10864595757746508No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTC5970.10071682713314697No Hit
GGTTTGAGGCCTGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT5940.10021071242393517No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGTCTTCTGCTTGAAAA5930.10004200752086459Illumina PCR Primer Index 12 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)