FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042746
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCT301862.894856465524682No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC155971.4957621510895271No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA107581.0316989947695796No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT91040.8730793501005998Illumina PCR Primer Index 10 (95% over 22bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG90130.8643523926248577No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTG66630.6389859083611925No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG49540.4750917289541269No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC42220.40489246662178513No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC41330.3963573104092464No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG40550.3888770611443247No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC33430.32059581144401417No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT31700.3040050021769443No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTT29900.2867428884886636Illumina PCR Primer Index 10 (96% over 28bp)
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAA26020.24953344342725842Illumina PCR Primer Index 10 (96% over 29bp)
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAT24240.2324631310021808No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG23090.2214345583680014No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT22540.2161600236299156No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG22270.2135707065766735No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTG19530.18729393351784615No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC19380.18585542404382277Illumina PCR Primer Index 10 (95% over 23bp)
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT18690.17923828046331514Illumina PCR Primer Index 10 (95% over 22bp)
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCC18280.17530635456765117No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCG18090.17348424256722156No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTT18020.17281293814601062No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA17250.16542858951269052No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTG15750.15104349477245657No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCT15060.14442635119194897No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG13200.12658883371405885No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG11240.10779230992015312No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATAT10610.10175057012925487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)