FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107931
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCT349973.1587707176710467No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTG130021.1735387853575718No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG106430.9606193887525486No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC102670.9266822572885856No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCG92020.8305571375834776No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA84570.7633146829540829No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT75620.6825334790704476TruSeq Adapter, Index 7 (95% over 21bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTT48320.4361282426432693No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATGT40940.3695175963124057No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC39000.3520074806102546No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCAT31790.2869312258615383No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG31680.2859383842495607No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCCG28930.26111734395011965No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT27910.2519109944572361No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT27780.2507376361885352No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTG26700.24098973672548202No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTG23530.2123778466348536No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC22610.20407408042558609No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCT22210.20046374729112193No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG21220.19152817278332315No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC20190.18223156496207796No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG18960.1711297905736007No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC18540.16733894078241335No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATGG17020.15361967487144956No Hit
GCAACTCATCCTTTGCACACGCATGTTTATAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCG16860.15217554161766392No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATGTCGTATGCCGTG16200.14621849194579808No Hit
GTATCAACGCAGAGTACATGGGGATCCGGAGGGATCACTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGC15580.14062247558737861No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATTGAAGTGATCCCTCC14990.13529723421404402No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA14330.12934018454217816No Hit
CGTCAGATGTGTATAAGAGACAGGCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGA13820.12473700979573638No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTTTGCCGTG13600.1227513265717811No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAAAAAAAAAAAAAAAAA12910.11652350191483044No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGGCGTG12220.11029567725787978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)