FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667200
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG610163.65978886756238No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC411052.4655110364683304No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG332741.9958013435700577No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC329311.97522792706334No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG277851.6665666986564298No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG171601.029270633397313No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC169081.0141554702495201No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG147820.8866362763915546No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA146410.8781789827255279No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC140690.8438699616122841No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG138920.8332533589251438No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG137670.8257557581573897No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC112240.6732245681381958No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA108310.6496521113243763No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC107810.6466530710172745No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC107590.6453334932821497No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC92890.557161708253359No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC92640.555662188099808No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC82750.49634117082533585No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT76850.4609524952015355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72670.43588051823416507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70840.42490403071017274No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA68780.4125479846449136No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT68030.40804942418426104No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT60850.36498320537428025No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC59090.35442658349328215No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG57370.34410988483685223No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC54320.32581573896353166No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG51810.310760556621881No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG51490.3088411708253359No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA50520.30302303262955854No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA47610.2855686180422265No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA47550.28520873320537427No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC45520.2730326295585413No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG45520.2730326295585413No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC45050.27021353166986567No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC44350.26601487523992323No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT42830.256897792706334No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT42350.2540187140115163No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG37050.22222888675623803No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG32480.1948176583493282No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC32260.19349808061420345No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG32030.19211852207293667No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA31620.18965930902111325No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC28700.17214491362763915No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG28290.16968570057581572No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG27610.1656070057581574No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG26860.1611084452975048No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26480.15882917466410748No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG25730.1543306142034549No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT24620.14767274472168906No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG23980.14383397312859883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.13795585412667946No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC22240.13339731285988485No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG21820.1308781190019194No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC21340.1279990403071017No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT20630.12374040307101727No Hit
CAAGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA19270.11558301343570059No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG19040.11420345489443377No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT19010.11402351247600767No Hit
TGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA18740.11240403071017274No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.11150431861804222Clontech SMART CDS Primer II A (100% over 21bp)
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC18480.11084452975047984No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC18300.10976487523992322No Hit
TGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG17640.10580614203454894No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA17480.10484644913627639No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC17290.10370681381957775No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC17210.10322696737044147No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGC16980.10184740882917466No Hit
CCAGCTCCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG16710.10022792706333972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)