FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295400
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG420713.2477227111316966No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC291922.2535124285934844No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC243541.8800370541917553No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG216371.6702948896093872No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG192801.4883433688436005No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC134851.040991199629458No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG116670.9006484483557201No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA114280.8821985487108229No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC111590.8614327620812104No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG106970.8257681025165972No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG102080.7880191446657404No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA99520.7682569090628377No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC99260.766249807009418No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG98830.7629303689979928No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC97630.7536668210591323No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC81690.6306160259379343No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT79630.6147136019762235No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC76860.5933302454840204No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC73670.568704647213216No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT67440.5206113941639648No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC58740.4534506716072256No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC55560.428902269569245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55190.426046008954763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.4106067623899954No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA48960.3779527559055118No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT45920.354485101127065No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC45430.35070248571869694No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC45360.35016211208893006No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG45070.34792342133703874No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC41120.31743090937162266No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT40820.31511502238690753No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG38700.2987494210282538No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG36010.2779836343986414No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA34860.26910606762389994No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA33820.2610776594102208No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC32880.25382121352478No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA31060.23977149915084145No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG31060.23977149915084145No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG29620.22865524162420875No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG29460.22742010189902734No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT28250.2180793577273429No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG26780.20673151150223867No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG23280.17971283001389532No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC22540.1740003087849313No Hit
TTTCCGATCCCTGGGCAAACTTCCGACCCGCTCTGGGCTGGATAGCACCA20490.15817508105604447No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA20200.15593639030415316No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC19890.15354330708661415No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG19870.1533889146209665No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT19420.14991508414389376No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG19110.1475220009263548No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG19080.14729041222788328No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18940.14620966496834953No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC18750.1447429365446966No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17990.1388760228500849No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG17880.1380268642890227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.132159950594411No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC16770.12945808244557666No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG16420.1267562142967423No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC15840.12227883279295969No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA15410.11895939478153467No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC15390.11880500231588698No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT14800.11425042457928053No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.10861509958314033Clontech SMART CDS Primer II A (100% over 21bp)
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG13950.10768874478925428No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA13880.10714837115948742No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG13810.10660799752972056No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT13490.10413771807935772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)