FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004046809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180354
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG33511.8580125752686385No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA32391.7959124832274305No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC26621.475986116193708No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC25981.4405003493130177No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21921.2153875156636393No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT21041.1665945862026903No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC17090.9475808687359304No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT14060.7795779411601628No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC13890.7701520343324795No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT12620.6997349656786098No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC11550.6404071991749559No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG9690.53727668917795No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG9330.5173159453075618No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG8530.4729587367066991No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA8230.4563247834813755No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC8090.44856227197622456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.44689887665369216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.4213934817081961No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7030.3897889705800814No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG6920.3836898543974627No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC6460.3581844594519667No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG6350.35208534326934804No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG6290.34875855262428335No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATC6170.3421049713341539No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC6050.3354513900440245No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC5880.3260254832163412No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG5750.318817436818701No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG5680.3149361810661255No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5230.28998525122814023No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC5180.2872129256905863No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT5020.27834148397041375No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA4610.2556084145624716No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA3780.2095878106390765No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG3670.20348869445645784No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATC3660.20293422934894706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.20016190381139312No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG3560.1973895782738392No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT3180.1763199041884294No Hit
GAAGTGCAGCGGCACAATCCCGGCTCACTGCAGCCTCAACCCCCTGCGCC2940.1630127416081706No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC2720.15081450924293335No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC2700.14970557902791176No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA2630.1458243232753363No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG2480.1375073466626745No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAT2330.12919037005001277No Hit
TTTTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATG2310.12808143983499118No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC2280.12641804451245883No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT2280.12641804451245883No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT2270.12586357940494805No Hit
TTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATG2250.12475464918992649No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTT2220.12309125386739411No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG2180.12087339343735098No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA2160.11976446322232942No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT2150.11920999811481864No Hit
AGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCC2140.11865553300730784No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA2120.11754660279228628No Hit
AACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAAC2050.11366534703971078No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG2020.11200195171717844No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.11089302150215687Clontech SMART CDS Primer II A (100% over 21bp)
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA2000.11089302150215687No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG1920.10645730064207058No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA1890.10479390531953825No Hit
GTGTATAAGAGACAGGGTTTGAGGCCTCAGGGGCTGTCTCTTATACACAT1810.10035818445945197No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)