FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781266
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG138101.7676437986550035No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC98031.2547583025499638No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA96031.2291588268272267No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC93541.1972874795524189No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC87511.120105060248366No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC78261.0017074850307066No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT67710.8666702505932677No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG58760.7521125967340189No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT55130.7056495482972509No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC45320.5800841198772249No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC42670.5461648145445981No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT40460.5178773938709735No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG37110.47499827203538875No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC36570.46808641359024966No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG36290.4645024869890665No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC35770.45784662330115483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.4501667805843336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.4461988618473094No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG34730.44453489592533146No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC33390.42738324719109755No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC32680.4182954333095258No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG31530.403575734768952No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG30620.39192797331510654No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC26260.33612111623953944No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC24520.31384957236075806No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC22560.2887620861524756No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG22520.28825009663802087No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC22490.2878661045021798No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG22410.2868421254732703No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC17720.22681135490345158No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT16600.21247564849871872No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC14980.19174007316330163No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC13980.178940335301933No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA12960.16588460268333705No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC12470.15961273113126645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.15961273113126645No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG12250.15679678880176534No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA11980.1533408595791958No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA11930.1527008726861274No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA11790.15090890938553578No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC11690.14962893559939894No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG11420.1461730063768294No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC10620.13593321608773454No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT10430.1335012658940745No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG10370.13273328162239237No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.13030133142873235Clontech SMART CDS Primer II A (100% over 21bp)
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC9960.12748538909923127No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG9770.12505343890557122No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT9630.1232614756049796No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG9380.12006154113963746No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG9070.11609362240261319No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT8910.11404566434479423No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA8820.11289368793727103No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG8680.11110172463667943No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT7970.10201391075510774No Hit
TTTTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATG7850.10047794221174351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)