FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973397
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG379973.9035460351737266No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC237232.437135105203735No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC225232.3138554978081913No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG170791.7545770122570752No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA127851.3134414837933546No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATC120911.242144777516265No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG102921.0573280994291125No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG98281.0096599845695025No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA89130.9156592839304004No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG88540.9095980365667863No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG85440.8777508046562709No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC85200.8752852125083598No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT82700.8496019609676216No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC81910.8414860534807482No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC77740.798646389910797No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT62420.6412594244691529No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC62370.6407457594383381No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC61530.6321161869206501No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC59060.6067411343984006No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT54500.559894883588094No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC45020.46250399374561457No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC43030.4420601255191869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43020.441957392513024No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT40830.4194588641633372No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATC40510.4161714079661228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.41452767986751554No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT36920.3792902587536226No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC34190.35124414807113646No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA31030.3187805181236433No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG29980.3079935524765332No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG29730.3054252273224594No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAT26670.27398892743659575No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC25850.2655648209312336No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT25490.2618664327093673No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG25330.26022270461076No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC25310.2600172385984341No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG24300.24964120497597586No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA21610.22200602631814154No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG20980.21553384692987548No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG19140.19663097379589212No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG19030.19550091072809964No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG18990.19508997870344782No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC17990.18481667808715252No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG16520.16971492618119843No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16300.16745480004561344No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAT16120.1656056059346803No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG16050.16488647489153962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.1636536788175842No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC15920.16355094581142124No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT15160.1557432373430368No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15000.15409950924442956No Hit
GTACATGGGCGTGCTGATGCACACCTGTAGTCCCAGCTAATTGGGAGACT14680.15081205304721507No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCA13930.14310707758499358No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC12960.13314197598718713No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC12590.12934085475915788No Hit
GTAGCTAGTAAAAAGGCCCTCAATTATAGTTATTTCCAGCTGGATTAAGA11900.12225227733391411No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC11590.11906755414286256No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATG11440.11752655905041828No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA10840.11136257868064109No Hit
CAAGGCCTGCTGCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCA10810.11105437966215223No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG10790.11084891364982633No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10660.10951338456970795No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA10610.10899971953889317No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATC10600.10889698653273022No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA10310.10591772935400459No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC10280.10560953033551573No Hit
GGTTTGAGGCCTCAGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10150.10427400125539733No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC10120.10396580223690849No Hit
CCAGCTCCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG9930.10201387511981237No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACA9870.10139747708283464No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT9830.10098654505818283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)