FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000299
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG405194.0506888440356335No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC260922.6084200823953636No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC248872.4879561011257634No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG203042.0297930918655323No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA120191.2015407393189437No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA117181.171449736528778No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC114401.143658046244173No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG112741.12706300816056No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG103361.0332910459772529No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATC99090.9906038094609712No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG94780.9475166925089398No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC92450.9242236571265192No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG89880.8985313391296003No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC88220.8819363010459873No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT80740.8071586595607914No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT76050.7602726784691377No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT66600.6658009255232685No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC62880.6286120449985454No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC62820.6280122243449209No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC55930.5591328192870332No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC51930.5191447757120621No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT46950.46935966146122315No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC43090.430771199411376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.42487296298406774No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC41080.41067720751495307No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT40410.4039792102161454No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG39990.3997804656407734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39310.39298249823302833No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATC34560.34549669648775017No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA31450.31440599260821017No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG31130.3112069491222125No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG27690.2768172316477373No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG26620.26612042999143254No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG25490.2548238076815032No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA23860.23852867992470253No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC22740.2273320277237106No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAT22650.22643229674327378No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT22230.22223355216790178No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT21250.21243648149203387No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC20950.20943737822391104No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC20640.2063383048468508No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT20320.2031392613608531No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG20100.2009399189642297No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT19560.19554153308160863No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG18930.18924341621855065No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA18630.18624431295042781No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17140.17134876671875107No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG16680.16675014170762942No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC16560.16555050040038027No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC16520.1651506199646306No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG16470.16465076941994344No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA16160.16155169604288316No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATG15450.1544538183083258No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA14920.14915540253464213No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG14200.14195755469114735No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA14120.14115779381964794No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGAT13790.1378587802247128No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC13710.1370590193532134No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.13226045412421686Clontech SMART CDS Primer II A (100% over 21bp)
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG13200.13196054379740457No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA13120.13116078292590516No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT12760.12756185900415776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.12686206824159577No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG12530.12526254649859692No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGA12150.12146368235897466No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG11700.11696502745679041No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACA11450.11446577473335473No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC11350.11346607364398045No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT11230.11226643233673132No Hit
CAAGGCCTGCTGCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCA11190.1118665519009816No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA10950.10946726928648333No Hit
GGTTTGAGGCCTCAGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10540.1053684948200488No Hit
GCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10500.1049686143842991No Hit
CCAGCTCCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG10500.1049686143842991No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACAC10480.10476867416642423No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG10380.10376897307704998No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA10180.10176957089830141No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10160.10156963068042656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)