FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906716
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG341133.762258524168538No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC235872.601365808036916No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC227562.509716383079156No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG161831.784792592167779No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC131081.4456566333890655No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC124861.3770574248165908No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG99911.10188857370996No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG99401.0962638797594837No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA95161.0495017182888577No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC90370.9966737104010517No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG85820.9464926173134698No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC81160.8950983549424517No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG80630.8892530847586235No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC78720.8681880544735066No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC74540.8220876217029367No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT63600.7014324220593879No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC61750.6810291204743272No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60320.6652579197896585No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC57310.6320611966701811No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT56440.6224661305193688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45100.4973994062087798No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT44900.4951936438752597No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC44780.49387018647514763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.4822899342241672No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC43500.4797533075406191No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC38550.4251606897859969No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC37590.41457303058510053No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC35500.3915228141998156No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG29420.3244676392608049No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA28270.31178450584306444No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT26780.29535157645833976No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC26770.2952412883416638No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG26110.28796227264104746No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT25020.275940867923363No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT24940.27505856298995496No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG24910.27472769863992696No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG23170.25553756633830216No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG23120.25498612575492213No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC21600.23822233202016949No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG21250.23436224793650934No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATG18870.2081136761676203No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG18160.200283219883624No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC17940.1978568813167519No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG16870.1860560528324194No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT16230.17899761336515513No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15650.17260090259794686No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG15490.1708362927311308No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC15440.17028485214775077No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT15300.1687408185142867No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15120.16675563241411864No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA14780.1630058364471345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.15539595639649018No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGA13500.14888895751260595No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG13060.14403628037886174No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATA12400.13675726467824545No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC12330.13598524786151342No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA12280.1354338072781334No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12260.1352132310447814No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT11810.1302502657943612No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11480.12661075794405305No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA11380.125507876777293No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA11020.12153750457695683No Hit
GGCTGGTCTGCAACTCCCGGGCTCAAGCGATACTCCAGCCCCGCCTCTCA10790.11900087789340874No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC10580.11668482744321264No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.1152510819264246Clontech SMART CDS Primer II A (100% over 21bp)
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG10360.11425848887634056No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG10290.11348647205960852No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC10160.11205272654282046No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC10130.11172186219279244No Hit
CAAGGCCTGCTGCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCA10070.11106013349273641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9860.10874408304254034No Hit
CCAGCTCCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG9770.10775148999245629No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG9700.10697947317572427No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG9690.10686918505904827No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACA9670.10664860882569624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9590.10576630389228822No Hit
GGTTTGAGGCCTCAGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAG9420.10389140590879614No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG9250.10201650792530408No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA9090.100251898058488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)