FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077781
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG415663.856627645133844No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC265022.4589411021348493No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC243402.258343763714521No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG204591.8982520567722014No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG159871.483325462222845No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC132101.2256664387292038No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG113201.0503061382599992No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG110181.0222856034760308No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA101680.9434198598787694No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG100320.9308013409032077No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG92920.8621417523597094No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC91850.8522139469892307No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA90340.8382036795972466No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC85330.7917192824887431No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC80690.7486678648074144No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC75510.7006061528269658No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC69830.6479052794584429No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC66510.6171012478416301No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT64960.6027198475386002No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT61090.5668127383949059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51600.4787614552492575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50390.467534684690118No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA48930.4539883334369413No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC48430.4493491720488671No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC45260.4199368888484766No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT38110.3535968809990156No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC37590.3487721531554184No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC37100.34422577499510565No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC35130.3259474791260933No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG33830.3138856595171004No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC33170.3077619664848425No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG32600.30247332250243786No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT32600.30247332250243786No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC32480.3013599237693001No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA32130.2981125107976481No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGA29810.2765868019569838No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA28540.2648033320312754No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG24200.2245354111827913No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG24140.2239787118162224No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATA22440.2082055630967701No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT22100.2050509333528797No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATG21300.19762827513196096No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG21200.19670044285434612No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG19720.1829685251456465No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG18610.17266958686412173No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC18370.17044278939784613No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18270.1695149571202313No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG17930.16636032737634082No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT17570.1630201311769274No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG17430.16172116598826664No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAA17340.1608861169384133No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG16710.1550407735894398No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT16590.15392737485630198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.15327789226197158No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC16190.15021604574584263No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC14690.13629856158162001No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA14660.13602021189833555No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.12293777678396632Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC13030.12089654577321367No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATGTC12920.11987593026783734No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA12530.11625738438513947No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT12120.11245327204691861No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC12100.11226770559139565No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA12010.11143265654154229No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC11770.10920585907526667No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG11730.10883472616422074No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11670.10827802679765183No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11670.10827802679765183No Hit
GCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11630.1079068938866059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11590.10753576097555996No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATC11470.10642236224242216No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT11390.10568009642033029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11040.10243268344867835No Hit
TGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAA11040.10243268344867835No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC10880.10094815180449461No Hit
GGTTTGAGGCCTCAGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10780.10002031952687977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)