FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327468
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG387862.921803011447357No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC288022.169694486044108No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240381.810815778610106No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG127590.9611531125420726No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC126810.9552772646873597No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG126250.9510587072532068No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA125470.945182859398494No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC124150.9352391168751337No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC123970.9338831519855847No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG109560.8253306294389017No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC107720.8114696550123996No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC102600.7728999870430022No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG91350.6881521814461817No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG91030.6857415771980944No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT84230.6345162369262385No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT81700.615457397089798No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC76610.5771137232686588No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC66730.5026863171089623No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC59370.44724241940295356No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT58490.44061325772071336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.4147745934365273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51700.38946324883161026No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC51620.3888605977695884No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT50260.3786155297152172No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT49380.3719863680329771No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC47630.35880337605124946No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC41880.3154878309684301No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA40720.30674939056911354No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG38300.28851919594295306No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG32440.2443750056498537No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG29450.22185092220678768No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC28090.2116058541524165No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA27940.21047588341112553No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG26760.2015867802463035No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG25040.18862978241283407No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT24900.18757514305429585No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC24760.18652050369575762No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG24350.18343191700289574No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG24270.1828292659408739No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT23370.17604944149312826No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC23150.17439215107256822No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG23130.17424148830706276No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC22280.16783832077308078No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC22220.1673863324765644No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC21250.16007918834954968No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG20900.15744258995320415No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG20790.15661394474292412No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT19630.14787550434360752No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA18800.141622999575131No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT18470.13913706394429093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.13785643043749454No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATA17780.13393919853435263No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC17360.13077528045873799No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17330.1305492863104798No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA17010.12813868206239248No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATG15220.11465436454965393No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA14390.1084018597811774No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG14370.10825119701567194No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA14320.1078745401019083No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.10689523212612281Clontech SMART CDS Primer II A (100% over 21bp)
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC14060.10591592415033733No Hit
GGTATCAACGCAGAGTACATTGGGGGGAATCAGGGTTCGATCTGTCTCTT13820.10410797096427184No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA13520.10184802948168996No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA13290.10011540767837718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)