FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071374
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG207901.940498836074051No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA156461.460367714728937No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC156211.4580342625451057No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC132511.236822995517905No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC132471.2364496431684922No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC118771.1085764634945408No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT112321.0483733971516949No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT96030.8963256528532519No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG91810.8569369799901808No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA87010.8121346980606212No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC76400.7131029873788238No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC75930.7087160972732212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69900.6524332305992119No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.6436594503880064No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT66160.6175247859290967No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC63660.5941902640907843No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG59910.5591884813333159No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC58010.5414542447361985No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG57890.5403341876879596No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG50000.466690436766246No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG49000.45735662803092103No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA47720.4454093528497052No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC45350.42328822614698514No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC44710.41731458855637715No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG42630.39790026638690135No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG39860.3720456161900513No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG36590.3415240616255388No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC36210.3379772143061153No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC35920.3352704097728711No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC28240.26358675868557574No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT27580.2574264449202613No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA27360.2553730069984898No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC26600.24827931235964282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22440.2094506680206912No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA20810.19423655978211157No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG19300.18014250859177094No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC18820.17566228039881499No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG18520.1728621377782175No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18420.171928756904685Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC18230.17015533324497328No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA18000.16800855723584854No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA17980.16782188106114204No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG17890.1669818382749628No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC17690.16511507652789784No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC17480.1631549766934796No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT17420.16259494816936007No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG16280.1519544062110897No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA16200.1512077015122637No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG16010.14943427785255195No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG15800.14747417801813373No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT15500.14467403539753623No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT14930.13935376441840105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14920.1392604263310478No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG14620.13646028371045033No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT14530.13562024092427108No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT14200.13254008404161385No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA13840.1291799128968969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13530.12628643218894614No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA12490.11657927110420824No Hit
AACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAAC12470.11639259492950176No Hit
GGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGT12360.115365875968616No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATG12310.11489918553184975No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA12250.11433915700773026No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA11590.1081788432424158No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCTG11290.10537870062181834No Hit
TTTTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATG11120.1037919531368131No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT10810.10089847242886238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)