FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837344
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG570223.1035015761882367No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC393962.1441820366790325No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG336481.831339150425832No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC330701.7998806973544421No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG275881.5015152306808088No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC195851.0659408363376701No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG164060.892919344445025No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG154690.8419218175801592No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG145960.794407579636693No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA145760.7933190518487556No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC143470.7808554086768728No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA139020.7566356653952662No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG137930.7507031889510076No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC135380.736824459654806No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC118070.6426123796088267No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC114540.623399864151732No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC103850.5652180538864796No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT98430.5357189508333768No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC95020.5171595520490447No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT88090.4794420641970148No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC85770.46681514185694134No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA69610.3788620965916018No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC68750.37418142710347113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65580.3569282616646638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65440.3561662922131076No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC59390.32323832662800217No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG58880.3204625807687619No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT57870.3149655154396781No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC56860.30946845011059443No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT56370.3068015570301479No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC54460.296406116655346No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA52940.288133305467022No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA51000.2775745859240295No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA49090.26717914554922756No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG48700.2650565163627497No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG43200.23512200219447202No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG41860.22782886601529165No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC41730.22712132295313234No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG41140.2239101659787171No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT39880.21705244091471168No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG36300.19756779351063272No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG32520.17699461831861646No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA30870.16801426406813313No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG29600.16110211261473084No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC29450.1602857167737778No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC28210.1535368444885661No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG27350.1488561750004354No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG26210.1426515666091924No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG26160.14237943466220807No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG26040.14172631798944563No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25710.13993024713934898No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT24800.13497744570423392No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT24720.134542034589059No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC23590.12839185258721283No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC21790.11859510249577652No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG20990.11424099134402703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.1120639357681523No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG20550.11184623021056482No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC20430.1111931135378024No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT19460.10591375376630616No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA19240.10471637319957502No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATA18770.10215833289792223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)