FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929523
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG355313.8224982060691346No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC244092.625970524666953No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC212082.2816003477052207No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG187972.0222199988596303No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC171741.8476143140083678No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATC142051.5282031751769456No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA120761.2991609675069902No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC117231.2611845000069928No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG102711.1049753475707433No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG100301.079048070892275No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC99841.0740992960905755No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC96041.0332181129461024No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG89410.9618912065650878No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG86430.9298317524149483No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT84510.9091759967208988No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG84100.9047651322237319No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC75080.8077261132860617No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT73590.791696386210992No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC64680.6958407699432935No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC62890.6765835810410286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59480.6398980982719094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58860.6332280104957059No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC58520.6295702204248845No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATC51970.5591039705311219No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC49910.5369420659843812No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT49680.5344676785835316No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC48660.5234943083710677No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG42740.4598057283144151No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC38540.41462126273368166No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG37570.4041858028257504No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT35990.38718783720252214No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC32480.34942653382433786No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC32290.3473824746671142No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA32220.34662940024076866No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG31240.33608635827193084No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG29380.3160760949433204No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG27890.3000463678682507No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG27460.29542033924927086No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC25420.27347359882434324No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC25390.2731508526416237No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT21700.23345307216712227No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC20810.22387826874644307No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT20600.22161904546740638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.21968256837108924No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT20390.21935982218836975No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG19640.21129116762038164No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTA18520.19924197679885275No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG17720.1906354119263321No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC17480.18805344246457592No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA17330.1864397115509783No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC16930.18213642911471797No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC16720.17987720583568131No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG16460.1770800722521121No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16410.17654216194757957No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA16030.17245404363313224No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG15060.16201858372520098No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG15030.16169583754248146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.15631673449715608Clontech SMART CDS Primer II A (100% over 21bp)
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC14410.15502574976627798No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATC14390.15481058564446495No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA14070.1513679596954567No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA13830.1487859902337005No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT13130.14125524597024497No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG13060.1405021715438994No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA12870.13845811238667574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12810.1378126200212367No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG12780.13748987383851718No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG12020.12931363720962258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11960.1286681448441835No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG11340.12199805706798003No Hit
CAAGGCCTGCTGCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCA11330.12189047500707352No Hit
CAAGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT11280.12135256470254098No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT11180.1202767440934759No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG11130.11973883378894336No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC11000.11834026699715876No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT10720.11532796929177654No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACA10330.11113226891642274No Hit
GGTTTGAGGCCTCAGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10160.10930337388101209No Hit
CTTCTCCAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA9990.10747447884560145No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC9960.10715173266288194No Hit
TGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTA9870.10618349411472335No Hit
AACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAAC9700.10435459907931272No Hit
CCAGCTCCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG9690.10424701701840622No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA9540.1026332861048086No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGC9480.10198779373936954No Hit
GCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG9410.101234719313024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)