FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042399
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG351373.370782205278401No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC228882.195704332026412No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC203061.9480064735288503No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG183771.7629525738224998No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG134071.286167772609145No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC123171.1816012870311656No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA122031.170664975695487No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC103780.995588061769054No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC100550.9646018463179646No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG96900.929586463532678No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG91800.8806608601888529No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG88750.8514014307381339No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG85300.8183046990643699No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT84900.8144673968413247No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA83180.7979669972822307No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT74180.7116276972637157No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC71790.6886998164810212No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC68420.6563705452518661No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC62030.5950696422387205No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC59860.5742522776787008No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC57350.5501732062290927No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT51640.49539571699512375No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC46460.4457026532066896No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC45630.43774025109387094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.43236802798160784No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC44910.43083310709238976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.41960899808998287No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA39420.3781661340810956No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG38230.3667501599675364No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC34290.32895273307054207No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG29630.28424816217206655No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG29570.28367256683860975No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA29220.2803149273934453No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG28380.2722565927250506No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT27340.2622796069451333No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA26620.2553724629436521No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC26430.25354974438770567No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGA26280.25211075605406374No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC23760.22793575204887953No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT22820.21891809182472355No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC22790.21863029415799518No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT19760.18956272981842845No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG19470.18678068570672074No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT19310.18524576481750268No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC18380.1763240371489228No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATA17800.17075994892550742No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG17690.16970469081417No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA17290.1658673885911249No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16470.15800091903388241No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG16290.15627413303351212No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG16030.1537798865885328No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG15740.1509978424768251No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG15520.14888732625415027No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC15180.14562561936456195No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC15090.14476222636437677No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG14750.14150051947478845No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATG14750.14150051947478845No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAA14690.14092492414133168No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA14500.13910220558538525No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC14350.13766321725174333No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA13860.13296252202851308No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG13760.1320031964727518No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG13340.12797402913855443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.1226977385818674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.12240994091513903Clontech SMART CDS Primer II A (100% over 21bp)
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG12220.11722958291402813No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT11920.11435160624674429No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA11650.11176142724618884No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATGTC10700.1026478344664567No Hit
GCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10630.10197630657742379No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA10610.10178444146627155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)