FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604805
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG350275.791453443671927No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC257394.255751853903324No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC224753.716073775845107No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG118741.9632774199948744No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG112691.8632451781979316No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC109121.804217888410314No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG101381.6762427559295972No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC100541.6623539818619224No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG81831.3529980737593108No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG81591.349029852597118No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC77881.2876877671315548No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC62631.035540380783889No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC61791.0216516067162142No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA59870.9899058374186722No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC57980.9586560957664041No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC53540.8852440042658378No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT51850.8573011135820636No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT41620.6881556865435967No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT38210.6317738775307744No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA36030.59572920197419No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC34250.5662982283545936No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT32990.5454650672530816No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC28420.4699035226229942No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG25720.42526103454832553No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC24650.40756938186688274No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA23200.38359471234530135No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT23020.3806185464736568No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG23020.3806185464736568No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC22950.3794611486346839No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG21610.3573052471457742No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC21500.35548647911310255No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG20580.34027496465803025No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC19730.3262208480419309No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT19540.32307933962186164No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT19470.3219219417828887No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC18530.306379742230967No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG17860.29530179148651214No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG17370.28720000661370193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17180.28405849819363266No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC16790.2776101388050694No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG16050.2653747902216417No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT15430.2551235522193104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.248179165185473No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14630.24189614834533446No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG13690.22635394879341278No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATA13280.21957490430800009No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG12830.21213448962888867No Hit
CAAGGCCTGCTGCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCA12430.20552078769190069No Hit
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACAC12100.20006448359388565No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACA12000.19841105810963866No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA11800.19510420714114468No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATG11550.1909706434305272No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA11170.18468762659038865No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG11100.18353022875141575No Hit
CCAGCTCCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG10890.18005803523449707No Hit
CCTCCGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGA10810.17873529484709946No Hit
GGTTTGAGGCCTCAGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10770.1780739246534007No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC10690.1767511842660031No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT10350.17112953761956332No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG10300.17030282487743983No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA10160.16798802919949404No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT9740.1610436421656567No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC9410.15558733806764163No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA9370.15492596787394286No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA9210.15228048709914765No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG9210.15228048709914765No Hit
CAAGGCCTGGGATCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9130.15095774671175008No Hit
GCAACTCATCCTTTGCACCTCAGACGTCACTGTCTCTTATACACATCTCC8670.14335198948421393No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC8140.1345888344177049No Hit
CTTCTCCAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA7820.1292978728681145No Hit
CAAGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA7720.12764444738386752No Hit
CTTCTCCAGCTGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCC7630.12615636444804523No Hit
CCAGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA7560.12499896660907235No Hit
GCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7540.12466828151222295No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA6750.11160622018667173No Hit
GCAACTCATCCTTTGCACCGCCTGGGATCTCCTGTCTCTTATACACATCT6740.11144087763824703No Hit
TGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA6710.11094484999297294No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC6590.10896073941187656No Hit
GCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC6520.10780334157290367No Hit
CCAGCTCCTCACCAACACCCTGGGTCTGTCTCTTATACACATCTCCGAGC6510.10763799902447897No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAG6320.1044964906044097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.10433114805598498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)