FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975815
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG317073.2492839318928284No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC204172.0923023318969274No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC201752.0675025491512224No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG130581.3381634838570835No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA114131.1695864482509493No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC113181.1598509963466435No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG111921.1469387127683015No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC97831.0025465892612841No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG91190.9345009043722426No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG87990.9017078032208975No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT81080.8308952004222112No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC80320.8231068388987666No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG77950.7988194483585516No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT74220.7605949898290147No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC73380.7519868007767866No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG72240.7403042584916198No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG65880.675127969953321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59180.6064674144176918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58770.6022657983326758No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC58080.5951947858969169No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC55940.5732643995019547No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC51520.5279689285366591No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC49090.5030666673498563No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC47110.48277593601246144No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC46810.47970158277952274No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT44560.45664393353248306No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG36760.37671074947607897No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG35690.3657455562785979No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG30530.31286668067205364No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG27130.27802401069874927No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC26240.26890342944103135No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA25120.2574258440380605No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG25110.2573233655969625No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG24150.24748543525155894No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT22380.2293467511772211No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATG22350.22903931585392723No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG21750.22289060938805No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGT21150.21674190292217274No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT21090.21612703227558502No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG20340.20844114919323845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.20710892945896509No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC19510.1999354385821083No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC19380.19860321884783488No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC19070.1954263871737983No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG17490.1792347934803216No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC15920.1631456782279428No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG15610.15996884655390622No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC15000.15371766164693101No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG14670.15033587309069854No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT14560.14920861023862106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.14900365335642515Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA14330.14685160609336811No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG14280.14633921388787835No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14120.14469955883031108No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA14110.14459708038921312No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC13980.1432648606549397No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT13900.14244503312615608No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG13520.13855085236443382No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATA13090.13414427939722182No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA12910.13229966745745864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12550.1286104435779323No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTC12390.12697078852036503No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA12370.1267658316381691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12270.1257410472271896No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG12090.1238964352874264No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA12080.12379395684632845No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA11410.11692790129276553No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG11390.11672294441056963No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG11380.11662046596947168No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG11130.11405850494202283No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT11040.11313619897214125No Hit
GTATAAGAGACAGGTTCTCGGTAGCTTTGTACGGCAGGCCCCTCATGTGG10030.10278587642124788No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT10020.10268339798014993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)