FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255945
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG389733.103081743229202No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC249521.9867112015255446No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC216021.7199797761844666No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG188801.5032505404297163No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG147011.1705130399818464No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC123600.9841195275270812No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA117220.9333211247307803No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC112380.8947844053680696No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG105650.8411992563368619No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG103030.8203384702355596No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC98820.7868178940956809No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG95350.7591892957096051No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG91450.7281369805206438No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG89270.7107795325432245No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT86820.6912723088988769No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT80700.6425440604485069No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC75950.604723932974772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73200.5828280697005044No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC72780.5794839742186163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69830.555995684524402No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC66820.5320296669042036No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC61740.49158203583755655No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC60120.4786833818359881No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC51980.413871626544156No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC51840.41275692805019326No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT50760.4041578253824809No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG44580.35495184900612686No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG42850.34117736047358765No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC40500.3224663500392135No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG32760.2608394475872749No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATG32630.25980437041430954No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG31430.25024981189462914No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA30900.2460298818817703No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG30660.24411897017783424No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG30230.24069525337494874No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC29870.23782888581904463No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT29560.2353606248681272No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG29430.2343255476951618No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG28020.22309894143453737No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGT27540.2192771180266652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.18949874397366126No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG22890.18225320376290363No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC22010.175246527515138No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT21850.17397258637918062No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT20740.16513461974847624No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTC20030.15948150595766533No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATA18820.14984732611698762No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC18710.1489714915860169No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.14610512403011278Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC18260.14538853214113676No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG17580.13997428231331788No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17310.13782450664638976No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC17230.13718753607841108No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG16610.1322510141765762No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA16560.13185290757158952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16410.13065858775662947No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA16330.13002161718865077No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG16320.12994199586765345No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT16300.12978275322565877No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA16300.12978275322565877No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG16180.12882729737369072No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA16030.12763297755873068No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG15450.12301494094088515No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC15380.1224575916939038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14340.11417697431018078No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA14140.11258454789023405No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG13280.1057371142844631No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG12740.10143756295060691No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA12680.1009598350246229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)