FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397684
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG595054.257400099020952No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC393542.8156579026446606No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC340372.4352428732102536No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG327262.3414448473331597No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG253331.8124983901940637No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC184161.317608271969916No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA169611.2135074881017454No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG167621.1992696489335215No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC164791.179021867603836No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG158911.1369522724736063No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG145161.0385752430449229No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG143321.0254106078341028No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC142271.017898180132276No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA140431.0047335449214556No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC126120.9023498873851313No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC120910.8650739365979722No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT118800.849977534263825No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC105980.7582543693710453No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT103630.7414408407050521No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC95070.6801966682025408No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC79480.5686550035630371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78740.5633605307065116No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC73370.5249398290314549No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73140.5232942496301024No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA70890.507196190269045No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC65730.4702779741343537No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT64740.4631948280154885No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC64420.46090532623969366No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG58770.42048131051081644No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG53770.38470784526402246No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC52360.37461972806442656No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA51420.3678943165980293No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT49420.3535849304993117No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA49260.35244017961141433No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG47870.3424951562728056No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA46080.3296882557144533No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC41850.2994239041156656No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG41500.29691976154839006No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG40940.29291313344074915No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG39730.284255954851025No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG39240.28075015525683916No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAT34040.24354575140017343No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG31210.22329797007048804No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT30820.2205076397812381No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT29970.2144261506892831No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC29020.20762919229239227No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC28670.2051250497251167No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG28610.20469576814215518No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC28280.20233471943586676No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA28210.20183389092241164No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG27310.19539466717798873No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG26700.19103030441787985No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC26460.18931317808603376No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25900.18530654997839283No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG25510.1825162196891429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24550.17564771436175847No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC23310.16677589498055356No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA23000.16455794013525232No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22660.16212534449847033Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA22470.16076595281909214No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG20290.14516872197148997No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC19700.14094745307236828No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA19540.13980270218447088No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA19260.13779938813065043No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG18910.13529524556337483No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG18290.13085933587277238No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATC17920.12821209944450962No Hit
CCTCCGCTCCACCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG17820.12749663013957377No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATG17770.1271388954871058No Hit
CAAGGCCTGCTGCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCA17630.12613723846019556No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACA17610.1259941445992084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG17500.12520712836377895No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT17420.12463475291983023No Hit
TGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAA17020.12177287570008671No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC16960.1213435941171252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16590.11869635768886244No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG16580.11862481075836885No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT16530.1182670761059009No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT16400.11733696600948426No Hit
GGTTTGAGGCCTCAGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAG16260.11633530898257403No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATA16210.1159775743301061No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG16080.11504746423368944No Hit
CCAGCTCCTCAGACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG15780.1129010563188818No Hit
CAAGGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA15580.11147011770901005No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGC15380.1100391790991383No Hit
GCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG14960.10703420801840759No Hit
GCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA14760.10560326940853583No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC14260.10202592288385644No Hit
CTTCTCCAGCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG14170.10138200050941414No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)