FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417726
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG454373.2049211201600305No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC304362.1468182145210006No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC277451.9570072073165055No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG241791.7054776451867286No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG195721.3805206365687024No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC157091.1080420335100012No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG133500.9416488094314416No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC124960.8814114998243666No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG124380.8773204413264621No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA121960.8602508524214129No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG114460.8073492339140285No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA111440.7860475155283884No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG111230.7845662702101818No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC103760.7318762581768269No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC102570.7234825347069885No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC95600.6743192972407926No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT87860.619724826941172No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT80470.5675990988385625No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC80010.5643544662367763No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC79360.5597696592994696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73220.5164608676147577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71930.5073617892314877No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC62970.4441619889879991No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC55030.3881568088615148No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA54660.3855469956818172No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC54020.3810327242358537No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC50970.3595193993761841No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT49130.3465408689690392No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC40810.2878553401715141No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG40570.2861624883792778No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC40430.28517499150047326No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA39960.2818598234073439No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC38780.27353663542884876No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT38510.2716321771625829No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG37270.2628857762360287No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA34890.24609832929635206No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG34640.24433494201277256No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGA31830.22451446894533922No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG31270.2205644814301212No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG29500.2080796994623785No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT29060.20497613784327862No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATA27110.19122171703135868No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATG25460.1795833609597341No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG24760.17464587656571157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.1697789276630322No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC23670.16695750800930503No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC23190.16357180442483243No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT22480.15856378453946673No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG22270.15708253922125995No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG22040.15546022292036685No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG21500.15165130638783517No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC21450.15129862893111926No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG21110.1489004222254512No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG21050.1484772092773921No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAA20120.14191740858247645No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT19580.13810849204994477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.13331207863860858Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA18620.13133708488099957No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC18000.12696388441772247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16360.1153960638374411No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC16220.11440856695863658No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA16100.11356214106251843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15590.1099648310040163No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA15280.10777823077237775No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATC14900.10509788210133691No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG14800.10439252718790513No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT14510.10234699793895295No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG14510.10234699793895295No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAA14300.10086575262074618No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)