FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345264
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG389122.8925177511625972No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC289072.1487975594381474No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC242281.8009847881159387No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC131760.9794360066128284No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG129530.962859334673343No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG127270.9460596581786176No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA122280.9089665671570786No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC120540.8960323029531749No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC119350.887186455595333No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG110770.8234071527967745No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC108310.8051207792671178No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC104630.7777655538243795No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG93830.6974839139380821No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG85720.6371983491716124No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT81630.6067953948072645No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT79480.5908134016817517No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC76410.5679926021955541No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC64150.4768580739542573No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC60390.4489081696975463No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT59520.4424410375955946No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55630.4135247802661782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.4027462267629254No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC50490.3753166664684404No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT47570.35361088975844146No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT46410.3449880469558392No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC45300.3367368784119697No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC42260.3141390834810119No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA40540.3013534889806016No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG36570.2718425528372126No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG31060.23088404952485161No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG29970.2227815506844753No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC29800.22151785820478362No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA28390.211036644108517No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG28150.20925260766659926No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT25680.19089189928519606No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG25570.19007421591598378No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC25290.18799284006707975No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG24900.18509378084896347No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG24600.18286373529656633No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC23750.17654527289810773No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG23260.17290286516252573No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC22390.166435733060574No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT22230.16524637543262882No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC22170.16480036632214942No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG22010.16361100869420425No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG21320.1584819039236908No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC20250.15052807478680763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.14681133219947906No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATA18590.13818848939687675No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT18290.13595844384447958No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA17750.13194436185016473No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT16800.12488255093424043No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA16650.12376752815804185No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC16620.12354452360280213No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16210.1204967946811927No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATG16030.1191587673497544No Hit
CCAGCTCCTCACCAACACGCATGTTTATAGCAGCCTGTCTCTTATACACA15550.11559069446591896No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15210.11306330950653551Clontech SMART CDS Primer II A (100% over 21bp)
CAAGGCCTGCTGCTCTGTGGATGATTGACTTGGCGCTGTCTCTTATACAC14520.10793420473602208No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG14490.10771120018078235No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAA14120.10496081066615921No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA13790.10250776055852233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)