FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443310
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG365342.531264939617962No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC238061.649403108133388No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC212311.4709937574048542No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG193811.3428161656193056No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA158611.0989323153030188No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC130170.9018852498770188No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG105520.7310972694708691No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG100970.699572510410099No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG89480.6199638331335611No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG89140.6176081368520969No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA87190.6040975258260526No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC79400.5501243669066244No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC75150.5206781633883227No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC66330.45956863043975305No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCCTT65940.4568665082345442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61760.42790530100948515No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC61320.42485675288053154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59420.41169256777823193No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC50490.349820897797424No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA48380.33520172381539654No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC45510.3153168757924493No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA45210.31323832024998094No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC39740.27533932419230794No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCCT38910.2695886538581455No Hit
CCCCTGGTCCGTGCCAGTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAG38730.2683415205326645No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG34800.24111244292632905No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT32950.22829468374777423No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT32240.22337543563059908No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA32210.22316758007635226No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT31890.22095045416438602No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC31720.21977260602365398No Hit
GTACATGGGCAGAAATCACATTGCGTCATCACCCCTTGGGGCCATCGCAA30920.21422979124373834No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG29430.20390629871614555No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCCTTGGGGCC29300.20300559131440923No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG29250.20265916539066453No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT27340.189425695103616No Hit
GTTCTAAGTTGATCGTTCTATGGCGGCCGAAGAGCGATCCGGGCCGTCCG27160.188178561778135No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAT26030.18034933590150418No Hit
CCCCTTGGGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCC24840.17210439891637971No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC24470.16954084708066874No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA22990.15928663973782486No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCCTTG22030.15263526200192612No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC21710.15041813608995988No Hit
CTACCTCCCCGTGCCGGGAGTGGGTAATTTGCGTGCCTGCTGCCTTCCTT21700.15034885090521094No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT20790.14404389909305695No Hit
GTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGAGCCTCCGGGT20270.1404410694861118No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT20230.140163928747116No Hit
GGATAAGGGCGCTCCCCCATCCGGGGGGGCCGCCTGGACCCTAGCTCGGT19420.13455182878245145No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.1342746880434557Clontech SMART CDS Primer II A (100% over 21bp)
GTTCTATGGCGGCCGAAGAGCGATCCGGGCCGTCCGCCGACCGAAGCCGA18960.13136471028399999No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATG18840.13053328806701261No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC18580.12873187326354008No Hit
TCCTTGCTGCGAGCCTCCGGGTGTCCTGTCCGTCGGCTTCGGTCGGCGGA18550.12852401770929323No Hit
GACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGGCCCC18480.1280390214160506No Hit
ACTTAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAA18240.12637617698207593No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG17990.1246440473633523No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA17900.1240204807006118No Hit
TCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCA17860.123743339961616No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC17850.12367405477686705No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG17640.12221906589713921No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG17610.12201121034289238No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC17360.12027908072416876No Hit
CCATAGAACGATCAACTTAGAACTGGCACGGACCAGGGGAATCCGACTGT16960.11750767333421094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16620.11515197705274681No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16260.11265771040178478No Hit
AAATCACATTGCGTCATCACCCCTTGGGGCCATCGCAATGCTTTGTTTTA16190.11217271410854217No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG15950.1105098696745675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.11037129930506959No Hit
GTTTTAATTAGACAGTCGGATTCCCCTGGTCCGTGCCAGTTCTAAGTTGA15890.11009415856607382No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATA15550.10773846228460968No Hit
CCCTAGCTCGGTCCCGTGCCTTGGCCACCCACGGATCTTCCTTGCTGCGA15450.10704561043712023No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCCTTGG15370.10649132895912868No Hit
GTCCTGTCCGTCGGCTTCGGTCGGCGGACGGCCCGGATCGCTCTTCGGCC15310.106075617850635No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG15060.10434348823191138No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14900.10323492527592826No Hit
GAGTACATGGGCAGAAATCACATTGCGTCATCACCCCTTGGGGCCATCGC14460.10018637714697466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)