FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247089
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG372422.9863145292757776No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC246331.9752399387694062No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC216481.735882523220075No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG195571.5682120522272267No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT161451.294614899177204No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATC141351.133439554033433No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA128531.0306401547924808No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC126581.0150037407113688No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC107120.8589603468557577No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG101990.8178245498116012No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC97540.7821414510111147No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG95040.7620947662917402No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC91670.7350718352900234No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG89730.7195156079477888No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG88880.7126997351432015No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT84970.6813467202420997No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT74970.6011599813646019No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC73910.5926601870435871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65910.5285107959415888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65670.5265863142085287No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC64810.519690254665064No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC64470.5169639055432291No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC61520.4933088175743672No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATC55880.4480834968474584No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT54220.4347724981937937No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC50950.4085514345808519No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC45340.3635666740705755No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG42860.34368036282895603No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC41700.3343787011191663No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC38290.30703502316193954No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAT34050.2730358458778804No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA33310.26710202720094556No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA33080.2652577322067631No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA32360.25948428700758325No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG32010.2566777511468708No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG30860.24745627617595856No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG30460.24424880662085863No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTA27660.22179651973515924No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG27340.2192305440910793No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT24300.19485377547231994No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAT23370.18739640875671265No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT21380.17143924772009053No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC21190.1699156996814181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.1651846820876457No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC19270.15451984581693848No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGA18470.14810490670673865No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG18420.14770397301235116No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG18160.14561911780153622No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT18150.14553893106265872No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC17630.14136922064102883No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG17620.14128903390215133No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC17120.13727969695827644No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA16700.13391185392542154No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.13294961305889155Clontech SMART CDS Primer II A (100% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT16480.13214774567011658No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16070.12886008937613916No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG15510.12436963199899927No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA15410.12356776461022428No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG15380.1233272043935918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14630.11731319897777945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14470.11603021115573948No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG14160.11354442225053704No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA14080.11290292833951708No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC14050.11266236812288456No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATC13990.1121812476896196No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT13900.11145956703972211No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG12990.1041625738018698No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA12760.10231827880768733No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG12550.10063435729125988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)