FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059103
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG299932.831924751417001No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC207431.9585441642597556No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC197851.8680902612871457No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG153781.4519834237085534No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC123601.1670253034879516No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC113841.0748718491024953No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC95600.90265063926738No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA94630.893491945542596No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG92300.871492196698527No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG86320.8150293219828477No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC79530.7509184659093592No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG74700.7053138363313106No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC74040.6990821478175399No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG73750.6963439816523983No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC69160.6530054206248117No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT67210.6345936136523077No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT56630.5346977583861059No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC55660.5255390646613219No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC54860.5179855028264484No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC53460.5047667696154198No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC45210.42687066319328715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44070.41610683757859246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41970.39627873776204964No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC40110.3787167064959687No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT38700.3654035537620043No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC38570.3641760999638373No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC33830.319421246092212No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC32050.3026145710096185No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG31140.29402239442244993No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA28660.2706063527343422No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT25490.24067536396365605No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG24390.230289216440705No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG24200.22849524550492256No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT23420.22113052271592093No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT23360.2205640055783054No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC23140.21848677607371522No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG22830.21555977086270173No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG21780.20564572095443032No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG20550.19403211963331235No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC18090.17080491699107642No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT18050.17042723889933273No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC16820.1588136375782148No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG16680.1574917642571119No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC16650.15720850568830416No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG16170.15267636858738007No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC15370.1451228067525066No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATG15230.14380093343140374No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG15000.14162928440387762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.1348310787524915No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT13840.1306766197433111No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC13820.13048778069743924No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13620.12859939023872088No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA13550.12793845357816946No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGA12910.12189560411027067No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA12490.1179299841469621No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA12440.11745788653228251No Hit
GGTATCAACGCAGAGTAAATGGGGGTGGGTGTTTTTCGTGATGTTCTTGT12310.11623043273411558No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11810.11150945658731964No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA11760.11103735897264005No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.10886570994511394Clontech SMART CDS Primer II A (100% over 21bp)
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG11460.10820477328456249No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATA11260.10631638282584414No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11210.10584428521116455No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC10690.10093447001849679No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG10660.10065121144968903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)