FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953898
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG197182.067097320677892No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC133721.401827029724352No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG127421.3357822324818798No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC122851.2878735462282132No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA91210.9561818978549069No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC90140.9449647656248361No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC76830.8054320273236761No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG73780.7734579588173998No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT67040.7028005090691039No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64070.6716651046547953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.6631736307236203No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT54920.5757428991359663No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG53130.5569777900781845No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG52900.5545666308137768No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG50700.5315033682846594No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC47830.5014162939853107No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC45290.47478870906532983No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG45240.4742645440078499No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG44130.4626280797317952No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC40070.42006587706442405No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT38360.40213943209861014No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC37540.3935431251559391No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC36920.3870434784431878No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC35510.37226202382225354No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC34020.3566419051093513No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC33910.3554887419828955No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG30940.324353337568587No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG25170.2638646899354019No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATC22020.23084229131416564No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG20980.21993965811858293No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG20470.21459317453228752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20360.21344001140583163No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT16850.17664362437073985No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATG16710.17517596220979603No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG16510.17307930197987625No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG16120.16899081453153272No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.1685714824855488Clontech SMART CDS Primer II A (100% over 21bp)
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15570.16322499889925338No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT15380.16123317168082962No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG15090.15819301434744595No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC14740.15452385894508638No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGT14690.15399969388760645No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA14370.15064503751973482No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTC13980.1465565500713913No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC13890.1456130529679274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13860.1452985539334394No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC13740.14404055779548758No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG13460.1411052334735999No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13020.13649258096777642No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAT12480.13083159834699307No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG12100.12684794391014553No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA11940.12517061572620972No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11920.12496094970321774No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA11530.12087246225487422No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG11420.11971929912841833No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG10680.11196165627771523No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC10650.11164715724322725No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG9990.10472817848449206No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA9910.10388951439252415No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG9860.10336534933504421No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG9700.1016880211511084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)