FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004046874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74738
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.6877358238111804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.5525970724397228No Hit
CGTCAGATGTGTATAAGAGACAGCAAGGCCTGGGATCTCCTGTCTCTTAT2280.3050656961652707No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGTCTCTTATACACAT1980.2649254729856298No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.2328132944419171Clontech SMART CDS Primer II A (100% over 21bp)
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA1590.21274318285209667No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT1480.19802510101956167No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC1440.19267307126227623No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTGGGATCTCCTGTCTC1420.1899970563836335No Hit
CCTCCGCTCCACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGC1220.16323690759720624No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC1160.15520886296127806No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCAGGGTCTGTCTCTT1130.15119484064331395No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC1090.14584281088602852No Hit
CGTCAGATGTGTATAAGAGACAGGGTTTGAGGCCTCTGGACTCCTGTCTC1070.1431667960073858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.13781476625010033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1020.136476758810779No Hit
CGTCAGATGTGTATAAGAGACAGCCAGCTCCTCACCGCCTGGGATCTCCT990.1324627364928149No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTT970.12978672161417218No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC940.12577269929620807No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT890.11908266209960128No Hit
CGTCAGATGTGTATAAGAGACAGGGCCCCTGTCTCTTATACACATCTCCG880.1177446546602799No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG830.1110546174636731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG830.1110546174636731No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC760.10168856538842355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)