FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003397
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG330053.289326159037749No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC229312.2853367111920804No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCC212132.114118339999023No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACG192671.9201771581936162No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC141871.413896991918453No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATC112641.122586573410126No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAG93700.9338277870075354No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACG91800.9148921114972439No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC84000.8371561804549944No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC79660.7939031111314863No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA79440.7917105592302947No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG79310.7904149603795905No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG77780.7751667585213031No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG77590.773273190970274No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGC72530.722844497242866No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC66790.6656388249117747No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC57430.5723557076610754No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCC56690.564980760357067No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT56170.5597983649542504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50350.5017954010227258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.48654719916443845No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT47260.47100001295598853No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATC45120.44967246264439703No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC39830.39695155556574313No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG38090.3796104632563183No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC34850.3473201534387685No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC32850.3273878634279353No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT30950.3084521879176438No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC30450.3034691154149355No Hit
CAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT29530.2943002620099522No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGA27680.27586289374993145No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG26720.26629539454473156No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAG26260.2617109678422399No Hit
GGTTTGAGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC25140.2505488854361733No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC24670.2458647972836275No Hit
GGGCGCTCGTTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG23670.2358986522782109No Hit
GGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC23210.2313142255757193No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC22290.22214537217073602No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT19870.19802730125762785No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG19220.19154930700410705No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG18580.18517097420064044No Hit
GGTTTGTGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACG18100.18038722459804046No Hit
GGGCGCTCGTTCTGGGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAG16780.16723191319089054No Hit
TGGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTA16540.16484003838959058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16370.16314579373866975No Hit
GCAACTCATCCTTTGCACCTCAGACGTCCCTGTCTCTTATACACATCTCC15620.1556711849846073No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG15320.15268134148298232No Hit
CCAGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG15190.15138574263227816No Hit
CGTCAGATGTGTATAAGATACAGGCAATCGCAGGCACTGATGACTCTCAC14780.14729962318005735No Hit
CTTCTCCAGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA14230.14181824342707822No Hit
GGTTTGAGGCCTCAGGGCCCCTGTCTCTTATACACATCTCCGAGCCCACG14140.1409212903765907No Hit
ATTAAATCCTATAAGGGGGCCAGGCGCAGTGGCTCACGCCTGTAATCCCA13650.1360378793239366No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.13374566597269077Clontech SMART CDS Primer II A (100% over 21bp)
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC13390.13344668162252826No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT13010.12965954652046996No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12980.12936056217030747No Hit
GGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATC12490.12447715111765334No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT12350.12308189081689501No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC12250.12208527631635334No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC11300.1126174385612076No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA10920.10883030345914928No Hit
CAAGGCCTGCTGCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCA10570.10534215270725346No Hit
CCAGCTCCTCACCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCT10550.10514282980714512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10520.10484384545698262No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC10480.10444519965676596No Hit
CAAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG10370.10334892370617015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10130.10095704890487014No Hit
GGTTTGAGGCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCAC10110.10075772600476182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)