FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459630
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCT16380.35637360485607994No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC15750.3426669277462307No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG15220.33113591366969086No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT8420.18319082740465156Illumina PCR Primer Index 10 (95% over 22bp)
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTT6040.13141004721188781Illumina PCR Primer Index 10 (96% over 28bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA5900.12836411896525465No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG5670.12336009398864305No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC4990.1085655853621391No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG4720.10269129517220374No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC4630.10073319844222527Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)