FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004046935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650003
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCT28190.4336903060447413No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG24920.38338284592532645No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC22120.3403061216640539No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT13440.20676827645410867TruSeq Adapter, Index 7 (95% over 21bp)
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCG12930.19892215882080544No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT10190.15676850722227434TruSeq Adapter, Index 7 (96% over 27bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA10070.15492236189679126No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG9490.14599932615695618TruSeq Adapter, Index 7 (96% over 28bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG8410.1293840182276082No Hit
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG8300.12769171834591533No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC8090.12446096402631988TruSeq Adapter, Index 7 (95% over 22bp)
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC7890.1213840551505147No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG7820.12030713704398288No Hit
CTTCTCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG7150.1099994923100355TruSeq Adapter, Index 7 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)