FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004046946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921249
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCT37200.40379962420583365No Hit
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG34810.3778565838334696No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC26790.2908008583998463No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCG16160.17541403030016858No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT15540.1686840365634047TruSeq Adapter, Index 7 (95% over 21bp)
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT12680.1376392267454293TruSeq Adapter, Index 7 (96% over 27bp)
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG11530.12515617384659306No Hit
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA11160.12113988726175008No Hit
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC10990.11929456639844385TruSeq Adapter, Index 7 (95% over 22bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG10300.11180473465914209No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC10200.11071925179837375No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTG10080.10941667236545169TruSeq Adapter, Index 7 (96% over 28bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG9940.10789699636037596No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)