FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004046967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004046967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549206
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG41160.2656844861174047No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCT34830.22482484575969885No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC30540.19713324115708306No Hit
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT18950.12232072429360588TruSeq Adapter, Index 7 (96% over 27bp)
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT18850.12167523234482697TruSeq Adapter, Index 7 (95% over 21bp)
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCG16140.10418240053291815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)