FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416986
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG67561.620198280038179No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC57281.3736672214414873No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCT35810.8587818296057901No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT34520.8278455391787735TruSeq Adapter, Index 11 (95% over 21bp)
GCAACTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC22610.5422244391898049TruSeq Adapter, Index 11 (95% over 22bp)
GGTTTGAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT22000.5275956506933087TruSeq Adapter, Index 11 (96% over 27bp)
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA17040.40864681308245365TruSeq Adapter, Index 11 (96% over 28bp)
GGTTTGAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG16270.3901809653081878No Hit
CCAGCTCCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC16180.38802261946444244No Hit
GGTTTGAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG13760.3299870978881785No Hit
GGTTTGAGGCCTCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC13730.3292676492735967No Hit
GGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA13650.32734911963471197TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG13140.3151184931868216No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT10060.24125510208975842No Hit
CAAGGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT9940.23837730763143125TruSeq Adapter, Index 11 (95% over 21bp)
CCAGCTCCTCACCGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA9850.23621896178768592No Hit
GGGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA9380.22494760015923798TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG8130.1949705745516636No Hit
CTTCTCCAGCTGCCTCTGGACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG7970.1911335152738941No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6720.16115648966631974No Hit
CAAACCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC6270.15036476044759298TruSeq Adapter, Index 11 (95% over 22bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG6220.14916567942329No Hit
GCAACTCATCCTTTGCACCTCAGACGTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATAT5630.1350165233365149No Hit
CTTCTCCAGCTGCCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG5590.1340572585170725TruSeq Adapter, Index 11 (96% over 28bp)
GCAACAGAGCCCGATAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCG5570.13357762610735133No Hit
CCTCCGCTCCACCCTGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT5140.12326552929834574No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA4970.11918865381571563No Hit
GGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA4960.11894883761085503TruSeq Adapter, Index 11 (96% over 28bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA4900.11750994038169148No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC4850.11631085935738851No Hit
CAAGGCCTGGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTT4850.11631085935738851No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA4680.1122339838747584No Hit
GGTTTGAGGCCTCAGGGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC4680.1122339838747584No Hit
GGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTG4470.1071978435726859No Hit
TGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAA4380.10503949772894056TruSeq Adapter, Index 11 (96% over 28bp)
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG4260.10216170327061341No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC4210.10096262224631043No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA4200.10072280604144983No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC4180.10024317363172865No Hit
TGTTTGAGGCCTCAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC4180.10024317363172865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)