FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156109
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG217821.8840784043719063No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC147711.277647695848748No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA109860.9502564204586246No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT101640.8791558581414036No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT100090.8657488178017817No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC97730.8453355176717766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84460.7305539529577229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83830.7251046397874249No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC69250.5989919635605293No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC65880.5698424629511577No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC50340.4354260714171414No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG48290.4176941793550608No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT47630.41198537508141536No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG41100.35550281158610475No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG40090.34676661110673823No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA36410.3149357024294422No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC34120.2951278815405814No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29830.25802065376188577Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA25970.22463279846450462No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC23980.20741988860911903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22460.1942723393728446No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC21300.18423868337674043No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT21160.183027724894452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.183027724894452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21110.18259523972220612No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA20430.1767134413796623No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA18770.16235493366109943No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG18540.16036550186876844No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA18080.15638663828410643No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG17180.14860190518368077No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC16630.1438445682889762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG15950.1379627699464324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14040.12144183636664017No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14010.12118234526329265No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA12680.10967823968155252No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG12450.10768880788922153No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT12410.10734281975142482No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG12400.10725632271697565No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA11960.103450453201212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)