FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626028
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC222083.547445162197218No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC99491.5892260410077506No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA79231.2655983438440452No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT68301.0910055141303583No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC66711.0656072891308375No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT61820.9874957669625002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.8504411943235766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52700.8418153820595884No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC45260.7229708575335289No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC41180.6577980537611736No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA40590.6483735551764458No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG39670.6336777268748363No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC35540.5677062367817414No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT32080.5124371433865578No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC28030.4477435514066463No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG26100.41691425942609595No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG24670.394071830652942No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22970.366916495747794No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA22920.3661178094270544No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC19400.3098902924469832No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.28624917735308963Clontech SMART CDS Primer II A (100% over 21bp)
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC15540.24823170848588244No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA15510.24775249669343863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15010.23976563348604216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14370.22954244858057465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.22874376225983503No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT14180.226507440561764No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14110.2253892797127285No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA13030.2081376551847521No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11130.17778757499664552No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10930.17459282971368692No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC10880.1737941433929473No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG10530.16820333914776975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10290.16436964480821945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10060.16069568773281706No Hit
TATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACA9060.14472196131802412No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG8610.1375337844313673No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA8260.13194298018618977No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT8260.13194298018618977No Hit
CTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTTGAT8040.1284287603749353No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG7910.12635217594101222No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA7900.1261924386768643No Hit
ATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACAG7830.1250742778278288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG7650.12219900707316605No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT7620.12171979528072226No Hit
AACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAAC7360.1175666264128761No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC7310.11676794009213645No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC6900.11021871226207135No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG6850.1094200259413317No Hit
GGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGT6770.10814212782814826No Hit
CCCCTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTT6570.10494738254518968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG6390.10207211179052694No Hit
CAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTA6280.10031500188489972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)