FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236303
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC266262.153679154705602No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC142441.1521447412163524No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA107280.8677484403095358No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC96670.7819280548538667No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT96060.7769939893375652No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT91030.7363081704080634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78400.6341487483246421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76120.6157066673784662No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC65640.5309378040820091No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC65520.5299671682427366No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG55990.45288250534051927No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC51580.41721163824725815No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA48650.3935119465050234No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC48460.3919751064261755No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT48210.38995294842769124No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA40000.32354527975747044No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG39720.32128046279916816No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG36590.2959630446581461No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC31150.25196088661113014No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG27100.21920192703568622No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.19889946073090498Clontech SMART CDS Primer II A (100% over 21bp)
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC21720.17568508690830645No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA21360.17277317939048922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21310.1723687477907924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG21290.17220697515091365No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT21090.1705892487521263No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC19010.15376489420473785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.15247071308570795No Hit
TATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACA17590.14227903677334763No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA17110.13839649341625798No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG16810.13596990381807694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG15750.127395953904504No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA15430.12480759166644423No Hit
CTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTTGAT15010.12141036622899078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14740.11922643559062786No Hit
ATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACAG14510.1173660502320224No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG14210.11493946063384138No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA14130.11429237007432644No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC13830.11186578047614543No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT13620.1101671677574187No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT13380.10822589607887387No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG12650.10232119472330004No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG12410.10037992304475522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)