FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004047226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924056
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT350891.200011217295428No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC153360.5244769594015983No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA149210.510284344759471No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT129250.4420229981915531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127550.43620915604899496No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC124710.4264966197637801No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG120400.4117568199788239No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCAAGTATTCGG118970.4068663527647898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118250.40440401962205924No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT101010.3454448204822343No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC86180.29472759755627115No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA80940.2768072841286213No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTTG80350.2747895389144394No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT77200.26401683141499344No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA77200.26401683141499344No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC77010.263367049057884No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC73490.2513289759156459No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC73160.25020040655856113No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG69410.23737575477350642No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC68810.23532381048789763No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG62180.21264982613192085No Hit
GTCCTGGGCCCAGTCTGTACTGAGTCAGCCACCCTCAGCGTCTGGGACCC61910.21172645120339692No Hit
AGTCTGTACTGAGTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG59840.20464724341804674No Hit
GTACTGAGTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC58900.20143253070392633No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT58550.20023556320398786No Hit
GTACATGGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATC57730.19743123934698928No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTTGACCACTCAGG56710.19394293406145435No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA52250.17869014820509593No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA50370.17226072277685517No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC50320.17208972741972112No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC49740.17010618127696597No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT48730.1666520750628579No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT48680.16648107970572382No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGAGTCAGCCACCCTCAGCGTCT47880.16374515399157882No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG46590.15933347377752No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC45820.1567001452776554No Hit
GTATCAACGCAGAGTACATGGGGAGCTTCAGCTGTGGTAGAGAAGACAGG44830.15331443720640098No Hit
CCCCTGAGGGCCGCTGATCATTACTATAGATGAGGAGTTTGGGGGCCGTT44180.15109149756365814No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT43670.1493473449208907No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT43390.14838977092093997No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA43170.1476373913495501No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC43100.1473979978495624No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC42630.1457906414925022No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT41990.1436019009211862No Hit
GTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGG41300.14124216499273612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41080.14048978542134624No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG40900.13987420213566362No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT40540.13864303556429836No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT40030.1368988829215309No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA39980.13672788756439685No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC39040.13351317485027644No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGAGTCAGCCAC38830.13279499435031342No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC38830.13279499435031342No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG38650.13217941106463077No Hit
GATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG37270.12745993920773063No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT36950.12636556892207262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG36930.126297170779219No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA36430.12458721720787838No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT36320.12421102742218343No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA36220.12386903670791531No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA35440.12120150913662393No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA35380.12099631470806306No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA35360.12092791656520942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG35250.12055172677951449No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34430.11774740292251586No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG34090.11658463449400422No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG34000.1162768428511629No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCGGAAGTAATTATGTATGCTGGT33800.11559286142262665No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC33440.11436169485126141No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG33420.11429329670840777No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG33100.11319892642274977No Hit
ACTCAGTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGA33050.11302793106561572No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG32800.11217295427994539No Hit
CATAATTACTTCCGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACC32620.11155737099426276No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA31920.10916343599438588No Hit
GTAATTATGTATGCTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTC31630.10817166292300831No Hit
TATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACA31540.107863871280167No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG31510.10776127406588655No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG31330.10714569078020394No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT30750.10516214463744882No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAG30680.1049227511374611No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC30600.10464915856604662No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC30480.10423876970892486No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG30430.1040677743517908No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG30330.10372578363752266No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC29420.10061366813768273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)