FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881776
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG668507.5812904864727555No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC122401.388107637313785No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA94101.0671644499283266No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC83600.9480865888842518No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT80310.9107755257571084No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT72250.8193690914699425No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA69660.7899965524124041No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG69050.7830786957231769No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC55030.6240813993576599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54930.6229473244905736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52080.5906261907786104No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG46570.5281386656021484No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC45150.5120348024895212No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT44830.5084057629148445No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC41610.4718885521946617No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG32770.3716363339442217No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG30210.3426040173468092No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG28230.32014933497849796No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC25640.2907767959209595No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA22160.2513109905463519No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC21060.23883616700840124No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA20170.22874290069133202No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC19760.2240931937362777No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA18160.20594799586289486No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA17880.2027725862350529No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT16740.18984413275026765No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.18133857124711944Clontech SMART CDS Primer II A (100% over 21bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC15860.1798642739199071No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG15740.1785033840794034No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA14670.16636878300157865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.16149226107310702No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA13880.15740959155159587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13740.15582188673767486No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG13600.15423418192375388No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC13280.15060514234907732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13240.15015151240224275No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG12060.13676942897062294No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC11760.13336720436936367No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11370.12894431238772658No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11210.1271297926003883No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA11120.12610912522001053No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT10480.11885104607065741No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG10430.11828400863711419No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA10410.1180571936636969No Hit
GTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTT10290.1166963038231932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10130.11488178403585492No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG10080.1143147466023117No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT9970.11306726424851662No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTCTG9730.1103454845675092No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG9640.10932481718713143No Hit
CTTCAATTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATC9560.10841755729346228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9490.10762370488650178No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC9160.10388125782511658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)