FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263633
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.8936665743666385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22250.8439762852146735No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA21660.8215966893370709No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC21500.8155276463872126No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT15340.581869492817667No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT15330.5814901776333008No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC14620.5545587995433046No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG14450.54811044140908No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC14170.537489616246828No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC11680.4430401353396578No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT11080.4202812242776891No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.32165927634249125Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC8010.30383146267728245No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACG7680.2913140615931996No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC7490.28410707309024286No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG7450.2825898123527783No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC6790.2575550101846127No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA6590.24996870649728978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.234796099122644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5770.21886486137926586No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA5730.2173476006418013No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5620.2131751336137737No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA5530.2097612969544784No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC5370.20369225400462004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5180.1964852655016633No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG4850.18396786441758048No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAA4740.1797953973895529No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCCTGTCTCTTATACA4590.1741056696240607No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4470.169553887411667No Hit
CATTACAAGCATGGGCCACCGTGCCAGGTGAACCAAAGGAAACCTTTCTA4300.1631055292774425No Hit
GAAAAGATAAAGACAAAGAAAGAAGAAAATAAAAACCCTAGAGAGTGGCT4010.1521053889308243No Hit
GGGATGAACGGAGGGTGCGGTGGGCAGAATAACGGCCTCCCAAAGATGTC3740.14186387895293837No Hit
TTTGTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTG3690.13996730303110763No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3660.1388293574780092No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG3590.1361741511874462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3570.13541552081871389No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT3500.13276031452815087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3470.13162236897505245No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA3470.13162236897505245No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC3450.13086373860632014No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG3440.13048442342195402No Hit
CCCAAGTGGTCTGCAGACTGCTGAGTGGGGATGGGATCTGCTCTCTGTTG3420.1297257930532217No Hit
CCTCTGGAGCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3280.12441538047209567No Hit
GCATGTGTCTGTGTTCGTGTAGGCATAATGTGAGGAGGTGGGGAGACCAC3220.12213948936589882No Hit
CTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTTGAT3200.12138085899716651No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA3130.1187256527066035No Hit
CCCAGAAGGAGGGAGGCAGGTCCAGGCATTGATGGTTAGTTTTGTTTTTT3020.1145531856785759No Hit
CCCCTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTT3010.11417387049420975No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT2990.11341524012547746No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT2910.1103807186505483No Hit
CAGCCGTAGGCCTCCCCGGCTTGCAGAGGCCGGCAGCCCTGTATCACCCC2800.1062082516225207No Hit
TTTTTTGGAGTGGGGGCAGCAGCCGTGTGATTTTCTGAGTGGAATGCCTT2670.10127715422576082No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAAAA2660.10089783904139465No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC2650.10051852385702853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)