FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12055
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGT1641.3604313562836998No Hit
GGATCAATGCGGACAAATGGCGGCCGCATAACTTCAAGCGCTTGACTTCG1341.1115719618415594No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT1070.8875985068436334No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCT840.6968063044379925No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGC710.5889672335130651No Hit
GCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCTCTTAT530.43965159684778105No Hit
CCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCTCTT510.42306097055163827No Hit
CATAACTTCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCGGCTGTCTCTT500.414765657403567No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT470.38987971795935294No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTACTGTCT450.3732890916632103No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCTGTCTCTTATACACATCTCCG370.3069265864786396No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC350.2903359601824969No Hit
GGATCAATGCGGACAAATGGCGGCCGCATAACTTCCAAGCGCTTGACTTC340.28204064703442555No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG340.28204064703442555No Hit
GCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTC330.27374533388635425No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGAAGTTATGCTG320.26545002073828283No Hit
CATAACTTCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCCTGTCTCTTAT290.24056408129406884No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC290.24056408129406884No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGATCAATGCGGA280.2322687681459975No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA260.21567814184985484No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT240.19908751555371215No Hit
CTTGGATCAATGCGGACAAATGGCGGCCGCATAACTTCAAGCGCTTGACT230.19079220240564082No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTACTGTCTCT220.18249688925756946No Hit
CTTGAAGTTATGCGGCCGCCATTTGTCCGCATTGATCCAAGCGCTTGACT210.17420157610949813No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT200.16590626296142677Illumina PCR Primer Index 7 (95% over 22bp)
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCGGCCG190.15761094981335547No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC180.1493156366652841No Hit
CCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGAAGTTATGCTGTC180.1493156366652841No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGATCAACTGTCT170.14102032351721278No Hit
CATAACTTCAAGCGCTTGACTTCGGCCGCCGCCGCGAGCGGCCTGTCTCT170.14102032351721278No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG170.14102032351721278No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC160.13272501036914142No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT150.1244296972210701No Hit
GGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGGAAGTTATGCGG140.11613438407299875No Hit
GACAAATGGCGGCCGCATAACTTCGGCCGCCATTTGTCCGCATTGATCCA130.10783907092492742No Hit
CATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATC130.10783907092492742No Hit
GCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTGTCTCT130.10783907092492742No Hit
AGGCCGCCGCTCGCGGCGGCGGCCGAAGTCAAGCGCTTGAAGTTATGCTG130.10783907092492742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)