FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016697
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT767607.549938673960875No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA113701.11832728925137No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC102231.00551098311493No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG88130.8668265963212246No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA76800.7553872982806087No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT66800.6570295771503211No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC65960.648767528575377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64480.6342105858480944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62440.6141456107375157No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT62220.6119817408726494No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC52740.5187386212411368No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG47260.4648385900617391No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC47200.4642484437349574No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC43780.4306101031083991No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG43240.4252987861673635No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA38630.37995587672630093No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA38480.37848051090934665No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC34950.3437602353503551No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT32900.3235969025186462No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC30420.29920418767833484No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG29230.28749961886383063No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG25900.25474649772744484No Hit
GGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACAC23870.2347798803379965No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.20330540957630444Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18780.1847158002826801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18760.1845190848404195No Hit
TATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACA18430.18127328004312004No Hit
CACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCA17710.17419152412173933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.1585526464620236No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT16090.15825757329863274No Hit
ATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTACAG15560.1530446140787275No Hit
GTGTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCC14810.1456677849939559No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14620.14379898829248047No Hit
CTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTTGAT14430.14193019159100498No Hit
GCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCAT14090.13858602907257522No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA13980.13750409414014206No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT13670.13445500478510314No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG13610.1338648584583214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12850.12638967165241954No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC12700.12491430583546524No Hit
CCCCTGTACGCCATGATGACCCTAAGGAAAAAGCCCATGTACTCTGCGTT11870.11675061498165136No Hit
GAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATAC11840.11645554181826051No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11690.11498017600130618No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT11540.11350481018435186No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC11520.1133080947420913No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC11470.11281630613643985No Hit
GGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCA11340.11153765576174612No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10990.10809513552218605No Hit
AACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAAATT10760.10583290793618945No Hit
GGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGG10470.1029805340234111No Hit
GTGGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGA10440.10268546086002024No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCTG10320.10150516820645679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)