FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004047301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906857
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA722823.7906355851539995No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA101780.5337579063348746No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG80080.4199580776114832No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC76820.4028618821442825No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA56280.295145362237441No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53740.28182501362189194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52280.2741684352838204No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT50560.26514835669376363No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT50460.2646239335199231No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC47290.2479997189091788No Hit
CACCTGCACCTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATATT39120.20515434560640888No Hit
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG39010.20457748011518428No Hit
CTCCCAGCCTGTGCTGAGTCAGCCACCTTCCTCCTCCGCATCTCCTGGAG38650.20268955668935845No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA37220.19519030530343912No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA35300.18512138036570125No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34930.18318101462249137No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC33340.1748426861584272No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCGGAGATGACTAAAATCC32880.17243033955876083No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC32090.16828739648542076No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG31980.1677105309941962No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC30170.15821847154768293No Hit
GACTATTACTGTATGATTTGGCCAAGCAAGATTTCTGGGGTATTCGGCGG29850.15654031739139326No Hit
GCCTTGGGCTGACGTAGGACGGACAGCTTGGTCCCTCCGCCGAATACCCC27630.14489812293213386No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26760.1403356413197214No Hit
GTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTG26650.13975877582849686No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26410.13850016021127962No Hit
GTATCAACGCAGAGTACATGGGGTGCATGTCAGGCTGTGTCCACCATGGC25990.13629758288114946No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCAT25850.13556339043777274No Hit
GTGCAGGTGAGTCTGGCGGATTCTCCAGGAGATGCGGAGGAGGAAGGTGG25780.13519629421608437No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCC25640.13446210177270765No Hit
GGTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTA25110.13168265895135292No Hit
GTGCTGAGTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAG24500.1284836775909258No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG24120.12649086953033184No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23290.12213815718745559No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22990.12056488766593405No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22690.1189916181444125No Hit
GTATTGGCTGAAGCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGA22660.11883429119226036No Hit
CTCCTGGAGAATCCGCCAGACTCACCTGCACCTTGCCCAGTGACATCAAT22350.11720857935335477No Hit
GGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCC22340.1171561370359707No Hit
CTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCC21840.11453402116676813No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT21480.11264609774094228No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21470.11259365542355824No Hit
CTCCAGAGCCCTGGCCCTTATCTGAGTCTGAGTAGTAGTACAGGAGATAC21430.11238388615402203No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT21410.11227900151925393No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCG21270.11154480907587723No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAAT21240.11138748212372507No Hit
TCCTCAGACTGGAGCCCGGAGATGACTAAAATCCCTGTATTGGCTGAAGC21180.11107282821942076No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21110.1107057319977324No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21070.11049596272819619No Hit
GGTTCCCTCTCCCAGCCTGTGCTGAGTCAGCCACCTTCCTCCTCCGCATC21030.11028619345865999No Hit
GATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGA20840.109289789428363No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20830.10923734711097896No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.10624813502008804Clontech SMART CDS Primer II A (100% over 21bp)
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT20240.10614325038531994No Hit
CTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCT20160.10572371184624753No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA19820.10394067305518977No Hit
GTACATGGGGTGCATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTT19630.10294426902489279No Hit
ATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCC19580.10268205743797253No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG19270.10105634559906694No Hit
CTATTACTGTATGATTTGGCCAAGCAAGATTTCTGGGGTATTCGGCGGAG19240.10089901864691478No Hit
GAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTG19170.10053192242522643No Hit
GGAGATACCTGGGAGGGCTCCCTGGCTTCTGCTGGTACCAATATATGTTG19090.10011238388615401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)