FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20723
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1480.7141823095111711No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC1170.5645900690054528No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA1150.5549389567147613No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT990.4777300583892294No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC890.4294744969357719No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA780.3763933793369686No Hit
CCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGAT430.20749891424986727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.1688944650871013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.1495922405057183No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT310.1495922405057183TruSeq Adapter, Index 12 (96% over 27bp)
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCTG270.13029001592433528No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT270.13029001592433528No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCATGAGACG220.10616223519760652No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT220.10616223519760652No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)