FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004047332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17162
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2251.3110360097890688No Hit
CGTCAGATGTGTATAAGAGACAGGCAATCGCAGGCACTGACGACTCTCAC1200.6992192052208367No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA1150.6700850716699686No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCTGTCTCTTAT970.565202190886843No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGCTGTCTCTTATAC640.37291690945111294No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA570.33212912247989745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.2622072019578138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.24472672182729288No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.16897797459503555Clontech SMART CDS Primer II A (100% over 21bp)
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCT280.1631511478848619TruSeq Adapter, Index 7 (96% over 26bp)
ACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG270.15732432117468825No Hit
AGCCGGAGGAGAATGCCGGCGGCCTGGATGAGCTCGCAGCCCAGGATGCG270.15732432117468825No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA260.15149749446451463No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC240.13984384104416733No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC230.1340170143339937No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT230.1340170143339937No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA230.1340170143339937No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC200.11653653420347278No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCT180.10488288078312552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)