FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004047337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730083
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT10492114.371105750989956No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA255043.4933014465478585No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG124631.7070661828860554No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA91951.2594458438287155No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG75721.0371423523078884No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC63170.8652440886858068No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG52170.7145762879015125No Hit
GAAACATCCTAAATGGATTTTCAAAGGACTCCATGATGTTCTGGGCTTTT50300.6889627617681826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39030.5345967513282737No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT35750.48967035254895674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.42707472985948175No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC30730.4209110471001243No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA29070.3981739062544944No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC23670.32420971314220437No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC23300.31914179620673266No Hit
CTGCTGTATGGGAAGAAATAGTAGGAGAATCAAATGATAAACTGCGAGGA23220.3180460303828469No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG22370.3064035185040605No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA19960.27339357305950146No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC19580.268188685396044No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA19480.26681897811618677No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19110.26175106118071506No Hit
CGGTTCGCCAGTGCGGAGCCGCTGCGGCGGCCGCTGCTACCGACTAGCAC18890.2587377051650292No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT18230.24969763711797152No Hit
ACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGC16930.23189144247982765No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC16630.22778232064025594No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT16390.22449502316859862No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA15850.21709860385736965No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA15750.21572889657751243No Hit
GGTCAGCACAGGGCTCTCCGTCTTCACCATGCACACCATGGCCGACACCA15620.21394827711369804No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA15240.20874338945024057No Hit
GCACCCTTCTTACCAGGAGTTGCTACCAATGCTTTGCCTGGTGTGGCTCC14990.20531912125059754No Hit
AGCCCATCTCGCCACCCCACCCCAGTAAACAGCCTCTCATCAAACGGGAC14740.20189485305095448No Hit
GGTCAGGCTGGTCGTGAACTCCTGACCTCATGATCTGCCCGCCTCAACCT14450.19792270193936856No Hit
GCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCC14190.19436146301173976No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.18354077550086773Clontech SMART CDS Primer II A (100% over 21bp)
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC13390.18340380477288198No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAAT13270.1817601560370533No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT13150.18011650730122467No Hit
GTATTGATCTGCGCAGTCAGGCCCTGCTCCTCGTCGTCCTGGCCGCTCAG12980.17778800492546737No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12950.17737709274151023No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC12870.17628132691762444No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12810.1754595025497101No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA12730.17436373672582434No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA12570.17217220507805278No Hit
CAACAAGGCTCCTGAAGGTCAAACCGGAGGAGAGACTCACCATCGAGGGA12520.17148735143812416No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT12390.16970673197430977No Hit
GATCTGCCACTCACACGGGGCCTCCATCCACAGAAGCCAGAGATGCCCAG12380.16956976124632403No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC11750.16094060538322355No Hit
ATGCTGAGAGGAGCCTGTGTGTCAAACCCCAGGGGAAAAAGGGACAGGCA11480.15724239572760906No Hit
GATGACCACAGGAAGTTCCGAAGACAGAGCCCGGAGACTGACATTGTAGT11300.15477692262386605No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11200.15340721534400883No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT11070.15162659588019445No Hit
GTCACTATTGCGGTTAGAAGTTGGCAGCATGGGAAGGGGGAGGACCAGGT11060.1514896251522087No Hit
ACACTACCTTGAACTCGGCATCAGAGGGAGACCTGTCTCTTATACACATC10740.14710656185666562No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG10560.14464108875292261No Hit
ATTAGCAACCACAAACTGGCCATAGATGAGATCTCCAACCTGCACATTTG10360.14190167419320818No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAAC9960.13642284507377928No Hit
GACGCGGCGCGCGTGGCCCGCTTCGTGACGCACGTCTCCGACTGGGGCGC9800.13423131342600775No Hit
CTACTAAAAATGCAAAAATTAGCCGGGCATGGTGGCACATGCCTGTAATC9730.13327251833010767No Hit
GTCCTAGACAATTGAGCCTTCCTACAAGGATGGTGAAGTCTTTTCCTTCT9690.1327246354181648No Hit
CCTCAAGCCCGCCTTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCTGC9600.13149189886629328No Hit
GTGCCACTGCCACCCGGGAACGTGTAGGGGTGCTGCTTCCGGAACACGTG9590.13135492813830757No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC9540.13067007449837895No Hit
ACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAAT9520.1303961330424075No Hit
TGCCCATCTCGCCACCCCACCCCAGTAAACAGCCTCTCATCAAACGGGAC9490.12998522085845035No Hit
GCAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAA9280.12710883557075017No Hit
AAACAGGGCATTCAGCAATTCTCGAAGACCAACCTGAAGAATATCTGCTC9120.12491730392297862No Hit
AGCCAGGTGAACCCCAGGGATGCCAAGGCCTGCGTAGTACACGGCAGTGA8770.12012332844347834No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG8650.11847967970764967No Hit
GAAGAGCATGTCAGTCCACTTCCACTTCAGAGAGGGTTGGTGGTGTCAGG8620.11806876752369251No Hit
GAATTTGTCAGTGTCCAGTATTAATTAGAAAACATCTGTCTTCTTACTTG8410.11519238223599235No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC8390.1149184407800209No Hit
ATTTTTAGTAGACGGGGTTTTGCCATGTTGGCCAGGCTCAAACTCCTGGC8300.11368570422814941No Hit
AGGGTGGCCTTTGTTGTCAGATCTCGGCTCAAGTTTCCGTCAGGAACCAT8210.11245296767627791No Hit
TAGCAATAGAGAGACACGTGCAAACAAAGCCAGATTTTAAATTGACTGCT8110.11108326039642068No Hit
GTGCCCCACAGTTCCACCCACGGCTACACCATAGGGGTCCTCTCTAGCTG8110.11108326039642068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8090.11080931894044924No Hit
CCCTTCACCATCCTGGGAGAAGGATGGAGGACAGAGGAAAGGGAGATGCA8090.11080931894044924No Hit
CTCGTGAGCCAGGCGCCCCAGATAGGCAAACTTTCTTGTAGGCTTCAGAC7980.10930264093260628No Hit
GTACATGGGGGTCCTCGCAGCCATGGCGGCCGCCGCGCTCCCAGCATGGC7910.10834384583670624No Hit
GTTCTCACTACAGCGCCAGGACGAGTCCGGTTCGTGTTCGTCCGCGGAGA7780.10656322637289184No Hit
GCGAAGGCTTCCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACT7740.10601534346094896No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC7720.10574140200497752No Hit
GGCCTCCCAAAGTGTGGGATTACAGGCATGAGCCACAGCGCCCGGCCAAT7640.10464563618109174No Hit
TTGATATCTGGCTGTCCACTGTCTCTTATACACATCTCCGAGCCCACGAG7390.10122136798144869No Hit
TTGATATCTGGCTGTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAG7350.1006734850695058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)