FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787991
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC740489.397061641566973No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA170332.161572911365739No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG90151.1440485995398426No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA59250.7519121411285155No Hit
CACAGTGCAGGGCGGTATGCCGCCAAACGCTTCCGCAAAGCTCAGTGTCC52060.6606674441713167No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG49630.6298295285098434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43690.5544479568929087No Hit
GACTTCCTGAGCCAGGAGGGGCTGGAGAAGAGCCCTGAGGAGATCCGCCA42730.542265076631586No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC37850.4803354353031951No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG36650.4651068349765416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35620.452035619696164No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA30530.3874409733106089No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC24230.30749082159567814No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC23940.3038105765167369No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG22360.2837595860866431No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC21320.27056146580354345No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA20090.25495215046872366No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA18020.2286828149052464No Hit
GGCTCCTGGTCCCGGCTCCCCGGTTACCGGGGCGCGAGTATGACCACAAT17920.22741376487802525No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC17460.2215761347528081No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC16820.2134542145785929No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16730.2123120695540939No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG16550.21002777950509588No Hit
GCTCATGCCTGAAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATCAC16070.20393633937443445No Hit
GAACAAAGGCCAACTTAAAGCCTGGAAAGTGATCTAATCTGTTAAATTAG16020.20330181436082392No Hit
GACTGCATTCTCCTCAGTTTCTCTGAAGCTGACTGTCACATTTTTTAATG15990.20292109935265756No Hit
GTCCACTGGCGAGTCTGAATGGAAGGATACTAATTATACACTCAACACAG15690.1991139492709942No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT15480.19644894421382986No Hit
GGACAGAACCTTCTATGACCCGGAGCCCATCCTGTGCCCCTGTTTCATGC14700.1865503540015051No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA14670.18616963899333877No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.1711948486721295Clontech SMART CDS Primer II A (100% over 21bp)
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13390.1699257986449084No Hit
CAGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCC13380.16979889364218625No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC13240.16802222360407668No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC13160.1670069835822998No Hit
TGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAATCCTATA12630.16028101843802786No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT12600.1599003034298615No Hit
ACGTAGAGCCTCCTGAAATGATTGGGCCTATGCGGCCCGAGCAGTTCAGT12390.15723529837269715No Hit
TAGTTGACCTTGGTCTGGATGAGGTCGAGCAGCGTGCTCACACAGCGCTC12270.1557124383400318No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12220.15507791332642124No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT12100.1535550532937559No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC11800.14974790321209253No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC11690.14835194818214928No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11480.14568694312498492No Hit
GAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC11300.1434026530759869No Hit
GTTTCAGCACCTCATTGAAACCCTCACAGAGCTTGATGTCACCCTGGTTC11070.14048383801337833No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC11010.13972240799704566No Hit
GGAGGGTAAGAAAGATTTGTATGTGAATGGCATTTCATAAGTGCTAGTCA10950.138960977980713No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA10710.13591525791538228No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA10690.13566144790993806No Hit
GTTTGTATCTGCACCTGACAGGGGTCACTCCCTGGGCTCGCCCTCCTCGT10530.13363096786638426No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA10480.1329964428527737No Hit
AGACATCACCATGCGCCCCTTTGTGCTGGAGGCCTTGAAGGGCTGCGGCT10380.13172739282555257No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT10200.12944310277655455No Hit
GAGTACATGGGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGG10150.128808577762944No Hit
GGTAACACTCTCAGCCAGAGAGGCAGACGCACGTGCAAACCACCCATTGC10080.12792024274388922No Hit
GCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCT9950.12627047770850175No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC9890.12550904769216906No Hit
CTAAGTGGCTTTTTTATTAGAGAAAGCCAGAATTACAAAAGACTTAAGAG9890.12550904769216906No Hit
GTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAG9840.12487452267855852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9840.12487452267855852No Hit
CAGTAATTGATATTACTGTGAAGGCCCTTAACTTATCAAGAAATGGTTGA9790.12423999766494795No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC9690.12297094763772681No Hit
GACCAAGATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGCCACT9560.12132118260233936No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9500.1205597525860067No Hit
GGCTAACACGGTGAAACCCCATCTCTACTAAAAGTACAAAAAAAAGAAAA9490.12043284758328457No Hit
AATCTCTTTTATTGGGGGAAATGGGCCTCTTGGGGGTCTCACTGCACGGC9280.11776784252612023No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAT9140.11599117248801065No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8960.11370688243901263No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC8900.11294545242267996No Hit
GAACAAGGATATGTCAAAGCCCCCAGGTTTCTTCAGGTGCATCAAGTCAA8880.11269164241723573No Hit
TTAAACTCCTATTCTAGAACATCGTGCTTGAATGCAGACCCCTCAGCCCA8840.11218402240634728No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA8830.11205711740362517No Hit
GAGTCAAGCAGGTGAGACAGAAACTACGTGGTCCACAAGCACAAAATGTT8640.10964592235190504No Hit
GGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGCACGGTGG8540.1083768723246839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.10672710728929645No Hit
GACATTTACAACAGCAGGCATTTTCTCTTCCTCTTCTTCACGGGAGGCGG8410.10672710728929645No Hit
TGCTTCCGGATAACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA8200.10406210223213208No Hit
GCTATGGCATCAAGACGCCCTGGGCGGATGACGGCCTGGCCGTGTCGCTG8180.10380829222668786No Hit
GTGATACTCCCGGATCACAATCTCGATGCGGCTCTTCCAGTACTTCCCTT8120.1030468622103552No Hit
GGCCTCCCAGAGTGCAGGGATTACAGGCATGAGCCACCACACCTGTCCTG7960.1010163821668014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)