FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132166
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT11930310.53758901079877No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA236832.091831056576509No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG143551.2679236083754502No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG80360.7097899071337596No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA78420.6926546107196295No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA64180.5668780019891075No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG59350.5242164134941343No Hit
GAGCAGAGCCTACGATGACTGGGGTCTCCTCCCCTTTATAGCCAAACTCG54120.47802177419212377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.4677759268517161No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC48720.43032558829712253No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT43490.384130948995112No Hit
GTGGTAAGAAGTGGGCTAGGGCATTTTTAATCTTAGAGCGAAAGCCTATA40920.3614310975598985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38830.34297090709312944No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA38690.34173433931066644No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT37550.33166514451061063No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGG37300.32945698775621246No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA31410.2774328146225907No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA30560.2699250816576368No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC27770.24528205227855282No Hit
GTCAGCAATGGTGATCTTCTTGCTGGTCTTGCCATTCCTGGACCCAAAGC27610.24386883195573794No Hit
GCTTCATCTCACCAAGCTGTTCATCTGAATTGGCAACACATTCAATTAGC26840.23706770915219144No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC26570.23468289985744142No Hit
CATCTGTAGTCTCAGCCACTCAGAAGCCTTAGGTGGGAGGATCCCTTGAG25680.2268218618117838No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG25460.22487868386791335No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22490.19864578162566265No Hit
TGTCAAAAGAGCAGTGGTTCCATTTCTAGATTGTGGATCTTCAGATAAAT21470.18963650206771798No Hit
GTCAGGTTGGGAAAACATGAGGAATGACTGACTCCAAAGCATGTGGTGGG20740.18318868434487523No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG20690.18274705299399557No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC20540.18142215894135666No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT20320.17947898099748624No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT19950.17621090900097688No Hit
GGATTCTGCTGGTTCATTGACACATTGGATCCTGAGCCAGGCCCAGCACC19810.17497434121851388No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT19670.1737377734360509No Hit
CACCCGAAGTGTCCGTGTTTCACCTTCCGGAGCCAGCGGCTGTGCGAGAA19510.17232455311323605No Hit
CTCCAAGCCTGAGGACTGGGACAAGCCCGAGCATATCCCTGACCCTGATG19370.17108798533077305No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC18970.16755493452373593No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA18550.16384523117634692No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA17790.15713243464297638No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17330.1530694262148837No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT17210.15200951097277254No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC16560.1462683034113372No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA15550.13734735012356844No Hit
TGTCATCAATGTCCACCAGGATGTAGCGGTCCAATGGCAGGGAGAGGCGC15330.13540417217969802No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT15280.1349625408288184No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCAT15180.1340792781270591No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.1318711213726609Clontech SMART CDS Primer II A (100% over 21bp)
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG14480.12789643921474414No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC14460.12771978667439227No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA14250.1258649350006978No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14150.12498167229893849No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT13560.11977042235855873No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT13380.11818054949539203No Hit
CTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAAT13330.11773891814451237No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13310.11756226560416053No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT13270.1172089605234568No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA13110.11579574020064196No Hit
GACAGGCCCAGTCCCGTCCTGCAGCAGTCTGCCTCCTCTTTCAACATGAC12910.1140292147971234No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA12890.11385256225677153No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC12730.11243934193395667No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA12660.11182105804272517No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA12510.11049616399008626No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC12100.1068747869128732No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA11990.105903197940938No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC11940.10546156659005834No Hit
ACCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11910.10519658777953056No Hit
GAGCAAGTTTATTTGGTGAATGCTGACGGCAAACATCATCCAAGAGAGAC11900.10510826150935462No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11670.1030767572953083No Hit
CAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAA11400.10069194800055821No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)