FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810808
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA8451710.423799469171493No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA162662.006146954642776No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG104221.2853844559994474No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG61700.7609693046935896No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA53750.6629189647857446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.6472555771526675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.5473552308314669No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG40250.4964183875837436No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA36400.44893488964095074No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC33450.4125514301782913No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA32110.39602470621898156No Hit
GTACATGGGATAGAAGCCGTAACGGAAGCAAGTGAAATGCTCAGTCTTAG31230.3851713352606289No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA29440.36309459206125244No Hit
CTCTTGCACCGGGCCTGCCTGCCTGTGAGGCAGAGCTGGGGTTGCTCAGA27190.335344495860919No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC26470.32646446507681226No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC25920.31968110822784185No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA22460.27700762695977343No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTA21760.2683742636974475No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTT21040.2594942329133408No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC20580.2538208799123837No Hit
GTGAAACAAGCATGAGTCAAGTCAACCAGGGAAGGAATCTGGGGACAGGC19190.2366774871486221No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18420.22718078756006352Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT17890.22064409823287387No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA17720.21854742429773757No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG17230.2125040700141094No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC16440.20276070290377005No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16020.19758068494637446No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG15770.1944973409241152No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA15130.18660398022713146No Hit
CCCAGGCTGCAGAAGACCATGGGACTTGGCCAAGGGGTGCGGTAGAATCT14800.18253396611774922No Hit
TCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA13980.17242059772473878No Hit
GCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTC13840.17069392507227357No Hit
ACCTGCTTTCATGCAAGATGCTGCTGCACTGCAAACGGGATGAGCTTCGG13620.16798058233268542No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT13340.16452723702775504No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC13080.16132055924460537No Hit
GTCCCCGATGGTATCATCCGTGTTGCATTTAACGCGGACCTTCTTCCCCA12600.15540053872186757No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG12600.15540053872186757No Hit
GCATGAGAGAGGATGCACAGCCTCTGGAGCCTGTCTCTTATACACATCTC12400.15293386350406016No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12400.15293386350406016No Hit
TGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC12130.14960385196002013No Hit
GATTACAAGTGCACACCACCACGCCCAGCTAATTTTTATATTTTTGGTAG12030.14837051435111642No Hit
ACAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA11900.1467671754595416No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11860.1462738404159801No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA11810.14565717161152825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11670.14393049895906307No Hit
CACCTCTTCCTGCCACATCCCTGACCGGTTCAACACCATCACCCGGTCTC11670.14393049895906307No Hit
CGTGGTTTTTCTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA11660.1438071651981727No Hit
GCATGAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA11220.13838047971899636No Hit
TACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGAT11150.13751714339276377No Hit
CGTGGTTTTTCTGCCTCAGCCTCCCGAACCAGAGCTGTCTCTTATACACA11120.13714714211009266No Hit
CGTGGTTTTTCTGCCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCC10950.13505046817495633No Hit
GCCAAATACTGCTCAGAAATATTAGGAGTTGCAGCTACTCCAGGCACAAA10610.13085712030468372No Hit
GCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCT10250.12641710491263036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10160.12530710106461704No Hit
GTCCAAGCTGGAACATGACTTCTGCTAATCAGATGTCATCAACATACGAA10150.12518376730372666No Hit
GAAGAGGAGGAGGACATGGCGGCGGCCGGAGGGACCGGCTGGCAGGCGGG10130.1249370997819459No Hit
GTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTC10030.1237037621730422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.12333376089037108No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9910.12222375704235774No Hit
CTACTAAATAATACAAAAATTAGCTGGGTGTGGTGGTGGGCGCCTACAGT9860.1216070882379059No Hit
ACGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA9840.12136042071612516No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC9740.12012708310722145No Hit
ATATATGGCTCTACAATCCTCAGCATGTTAATCGAAGCCTTGTTGAGCTT9720.1198804155854407No Hit
TCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA9410.1160570689978392No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9400.11593373523694883No Hit
CGTGGTTTTTCTGCCTCAGCCTCCTGAGTAGCTAAGGGAGGTGTCTTGAG9260.11420706258448363No Hit
GTATCAACGCAGAGTACATGGGCTTTTTCCTTAGGGTCATCATGGCGTAC9090.11211038864934733No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCTGTCTCTTATACAC8970.11063038351866288No Hit
GCGCCACAGAGGTCACAGGGCACCGCTGGCTGAAGGGGGGCGTGGTGCTG8880.10952037967064951No Hit
GCGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTAT8810.10865704334441692No Hit
GTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCG8700.10730037197462285No Hit
GGTAGAGACAGAACGAAGAGCTGGAAATAAAGAACAATCATTTGAATCAA8700.10730037197462285No Hit
CTGCAGTTGATCATGGTTCCGATGAAGTTAAATTCCGTCAAGATTTAATG8600.10606703436571914No Hit
TACCACCTCTTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATG8510.1049570305177058No Hit
CTACAGTACAAGCTGCTGGAGCCAGTTCTGCTTCTCGGCAAGGAGCGATT8490.10471036299592505No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC8430.10397036043058283No Hit
GGGAAGCTTGTCTGCCTTCAGAATCATCAGTGGAGCTTTTGAAAGGGCAG8390.10347702538702135No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC8360.10310702410435023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)