FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004047345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004047345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068860
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACA519574.860973373500739No Hit
GCCACAGCTTCTTCATGAGTAGCAAACTCAACATCTGCTTCACCCGTCAC117881.1028572497801397No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA108891.0187489474767508No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG84850.7938364238534513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76720.7177740770540576No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG64130.5999850307804577No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA62360.5834253316617705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62060.5806186029975862No Hit
GTACATGGGGAAACGGTCGTACACTTCGGGGCTGCGAGCGCGGAGGGCGA58920.5512415096457908No Hit
GCGCTAAACCATTCGTAGACAAACCCTTGTGTCGAGGGCTGACTTTCAAT53970.5049304866867504No Hit
GGAAATGACATTGAGCTTGTTTCAAATTCAGCGGCTTTGATTCAGCAAGC45930.4297101584866119No Hit
GAATGTGTAATACAAGGGCCAGAGCTTCCTCCTGGACTCAATTTTATAAA38630.3614130943247946No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA36090.3376494583013678No Hit
TTGATATCTGGCTGTCCTCTGTCTCTTATACACATCTCCGAGCCCACGAG36010.336900997324252No Hit
TGCTTCCGGATGACTGACGACTCTCACCTGTCTCTTATACACATCTCCGA35410.33128753999588345No Hit
CGACCAGGCCATGATCGATGCGCGCAACCTTGTCTTCGTTGCGGGCAAGC34630.32399004546900434No Hit
CTAGGGGTCAGGTCTCAACAGATGTCTGGGCTTAAGGGAGTTAAGTCTAT34620.32389648784686487No Hit
CCCTAAAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGT33060.30930149879310664No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTC30330.2837602679490298No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26650.24933106300170277No Hit
GGTTAAGTACAAGGCCATTGTTTTCCGGCCATTTAAAGGGGAGGTCGTGG26150.24465318189472898No Hit
TCCATGAGGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG26070.24390472091761314No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC25590.2394139550549183No Hit
GTCCTGAGGTGTCCCTGGTACTGCACTCCTGAGTTTCTCCCTGAGTTGTC22870.21396628183298094No Hit
CTTTTATGCTTTGCTTCCCTTATTTATAAGGGAATGCCTTTAAAAGCACC22780.21312426323372566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.21087888030237822Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCT22380.2093819583481466No Hit
GTCCCTGGGCAAACTTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAA22260.2082592668824729No Hit
CCATCATGGTGGGCTGTGCCAAGAAAGGAGAACGAGAGTGCCACATTGTT20550.19226091349662258No Hit
CGTGGTTTTTCTGCCTCAGCCTCTGGAGCCTGTCTCTTATACACATCTCC20500.1917931253859252No Hit
GTCCAGCCTGTTGTAAAAATTTGTTGTTGCTTCTTTCGTATTAGTTTTTC19630.18365361225979082No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG19410.18159534457272236No Hit
GCATCAGAGGGAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACA18490.17298804333589057No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATA18240.17064910278240367No Hit
GGCAGTGGCTGATTGACACCCTCTATGCCTTCAACAGTGGCAACGTAGAG18180.17008775704956683No Hit
GTCCTCCAGCAAATCAAAATCTGGTAGCATCAGATGTCTGTGCACTGTGA17410.1628838201448272No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGA17350.16232247441199035No Hit
GTGGAATGCGTACCCCACAATCAGTGCCGTGTAAGGCAGGAAGGTGATGG17320.1620418015455719No Hit
GAATCCTTGCCCTTCTTGTACTGTGTCACTTTATGGGGTTGGTGCTTGCC17090.15988997623636397No Hit
GTACAAGTACATTGTGATTGAGCCCACTCGCATTGGCGACGAGACAGCCC16980.15886084239282974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16620.1554927679958086No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATT15840.14819527346892952No Hit
GACTATTTGCTGACTAGTTCCAAAGACAGTGTGAGTCAAAGCAAACTGGG15760.1474468124918137No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGC15450.14454652620548997No Hit
TGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTA15400.14407873809479257No Hit
CCTTTGGCCATTGGGAGTTTGGCTGTCCCTCAGAGCCATCCGGTCAAGCA15380.14389162285051363No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15310.1432367194955373No Hit
GTAATCCCAGCTACTCAGGAGGCTGAGGTGGGAGAATCGCTTGAATCCAG15020.14052354845349252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.13837172314428456No Hit
CCTCTGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG14680.13734258930075033No Hit
GGCATGGAATTTAATGGGTGTTCTGGACAGACACTTGTACATGGACAGAC14300.13378739965945025No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG13830.1293901914188949No Hit
ATTCAGCACGTGGGCCAGCCCCCGGCCACTCCTGCCAAGCACAGTAGCAG13820.1292966337967554No Hit
CGTGGTTTTACTCCTACCACTGTCTCTTATACACATCTCCGAGCCCACGA13510.12639634751043166No Hit
AACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAAT13290.1243380798233632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12960.12125067829276051No Hit
GTTCCTCGCTCCGGAGATTCTTCAGCAAGGCCAGGTCGTCGGGGGCCGCG12830.12003442920494732No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12810.11984731396066836No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAAC12750.11928596822783151No Hit
TTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAG12710.11891173773927362No Hit
ATCTCAGGGTGACGGGCTCGGGTAGCCCCTCATGCTGCACATGGCACGTG12650.11835039200643677No Hit
CTCTCCCCCTGGGCCACTCCCGGGGGTGAGGGGGTTACCCCTTCCCAGTG12470.1166663548079262No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT12320.11526299047583406No Hit
ACGCAGAGTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACC12310.11516943285369459No Hit
ATCTTGGCATCCAGTCTTGCATTTCTAATCAAATTTACAATCCACCTTTC12280.11488875998727616No Hit
GAAGAAAGATTGTCCCCAGGCACAGTATCTGAATCACTGGGGATTATGAT12240.11451452949871826No Hit
CAGAAGCGAGATGACGAAGGGAACGTCATCGTTTGGAAAGCGTCGCAATA12100.1132047227887656No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC12010.11236270418951032No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA11950.11180135845667347No Hit
GAAAGATACTCTCTGGAAAGGCTCCTGTTTTCCCCTTGTGTCACAGGCAC11920.11152068559025503No Hit
CTGGGGGAGTGCATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTA11850.11086578223527871No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA11710.10955597552532603No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACC11470.10731059259397864No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA11450.10712347734969968No Hit
TCTGTGGCATGATAAATTAGCACTGTAATGCTAGCCTGTTCTCCAGTGAT11400.1066556892390023No Hit
GATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC11340.10609434350616544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11330.10600078588402598No Hit
ACGCAGAGTACATGGGGATGACCTCTACGTTGATCATGGTGTGTCTGGGG11220.10497165204049173No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC11110.10394251819695752No Hit
ACCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAG11080.10366184533053908No Hit
AGATGGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCC10840.10141646239919166No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACCTGTCTCTTATACACATCTC10820.1012293471549127No Hit
CTTTTGTCCTCTACACACACGTGCAGGCGTTAAGGAGGCAAGCAGGTGAG10740.10048088617779691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)